Bone protective effects of purified extract from Ruscus aculeatus on ovariectomy-induced osteoporosis in rats
| Autor: | Aleksandar Shkondrov, Lidija Chakuleska, G. Marinov, Reneta Petrova, Irini Doytchinova, Rositza Michailova, Rumyana Simeonova, N. Zlateva-Panayotova, Vassil Manov, Mariyana Atanasova, Ilina Krasteva, Nikolay Danchev |
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| Rok vydání: | 2019 |
| Předmět: |
medicine.medical_specialty
Cell Survival medicine.drug_class Ovariectomy Osteoporosis Toxicology Bone and Bones Cell Line Bone remodeling 03 medical and health sciences chemistry.chemical_compound 0404 agricultural biotechnology Ruscus aculeatus osteoporosis Ruscus aculeatus ovariectomized rats molecular docking oestrogen receptors vitamin D receptor Internal medicine medicine Animals Rats Wistar Receptor Cell Proliferation 030304 developmental biology 0303 health sciences Osteoblasts biology Plant Extracts business.industry 04 agricultural and veterinary sciences General Medicine Diosgenin biology.organism_classification medicine.disease Glutathione 040401 food science Rats Ruscus Endocrinology chemistry Estrogen Ovariectomized rat Calcium Female Bone Remodeling business Food Science Hormone |
| Popis: | Ruscus aculeatus is a source of steroidal saponins that could mimic sex hormones and could help alleviate the risk of fracture in osteoporotic patients. The aim of the present study was to evaluate the in vitro effects of an extract from R. aculeatus (ERA) on the proliferation of human osteoblast-like SaOS-2 cell line and to investigate the effects of the ERA administered orally for 10 weeks at three doses (50, 100 and 200 mg/kg) on the bone structure of rats with estrogen deficiency induced by bilateral ovariectomy. Bone turnover markers, hormones, histopathological and radiological disturbances were evidenced in the ovariectomized rats. ERA recovered most of the affected parameters in a dose-dependent manner similar to diosgenin and alendronate used as positive comparators. The main active compounds of ERA (ruscogenin and neoruscogenin) were docked into the Vit. D receptor and oestrogen receptors alpha and beta, and stable complexes were found with binding scores equal to those of estradiol and diosgenin. The findings of this study provide for the first time an insight into the effects of ERA on bone structure and suggest that ERA could be developed as a potential candidate for the prevention of postmenopausal osteoporotic complications. |
| Databáze: | OpenAIRE |
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