Synthesis and characterization of a collagen-based composite material containing selenium nanoparticles
| Autor: | Magdalena M Stevanović, Nenad Filipović, Maja Kuzmanović, Nina Tomić, Dušan Ušjak, Marina Milenković, Kai Zheng, Juergen Stampfl, Aldo R Boccaccini |
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| Rok vydání: | 2022 |
| Předmět: |
collagen
composite materials dimethyl sulfoxide health risks bone tissue antibacterial agents light scattering pseudomonas aeruginosa biofilm synthesis (chemical) calcium phosphate Field emission microscopy antibacterial activity Candida albicans Enterococcus faecalis bacteria bone tissue engineering inductively coupled plasma mass spectrometry mesenchymal stem cell MTT assay extracellular matrices quantitative analysis photon correlation spectroscopy biomaterial Fourier transform infrared spectroscopy stereolithography field emission scanning electron microscopy 3D printers Anti-Bacterial Agents Klebsiella pneumoniae triphenyltetrazolium scaffolds tissue engineering cytotoxicity MRC-5 cell line Collagen ddc:620 scanning electron microscopy (SEM) differential scanning calorimetry escherichia coli thermal analysis biomaterials staphylococcus aureus Staphylococcus aureus X ray diffraction extracellular matrix Biomedical Engineering collagen gel Salmonella enterica serovar Typhimurium sodium selenite Biomaterials zeta potential Selenium biocompatibility bacillus subtilis selenium nanoparticles cell viability antimicrobial activity human cells scaffold protein colorimetry Nanoparticles nanoparticles bone metabolism biofilms multidrug resistants |
| Zdroj: | Journal of Biomaterials Applications |
| ISSN: | 1530-8022 |
| Popis: | Multidrug-resistant bacterial strains represent an emerging global health threat and a great obstacle for bone tissue engineering. One of the major components of the extracellular matrix of the bone is a collagen protein, while selenium is an element that has antimicrobial potential, and is also important for bone metabolism and bone health. Here we represent the incorporation of selenium nanoparticles (SeNPs) synthesized by the green chemical reduction method into collagen gels to produce a composite material, collagen/SeNPs, with antimicrobial properties. The samples were comprehensively characterized by zeta potential measurements, dynamic light scattering inductively coupled plasma-mass spectrometry (ICP-MS), X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), optical microscopy, field-emission scanning electron microscopy (FE-SEM), and differential scanning calorimetry The cytotoxicity of the SeNPS, as well as collagen/SeNPs, was tested on the MRC-5 cells. It was revealed that collagen/SeNPS expressed a lower cytotoxic effect. Collagen/SeNPs showed significant antibacterial activity against all tested Gram-positive strains, the major causative agents of orthopedic infections as well as Candida albicans. Furthermore, three-dimensional β-tricalcium phosphate (3D-TCP) scaffolds were fabricated by a well-established 3D printing (lithography) method, and afterward preliminary coated by newly-synthesized SeNPs or collagen/SeNPs. In addition, uncoated 3D-TCP scaffolds as well as coated by collagen/SeNPs were subjected to biofilm formation. The production of Staphylococcus aureus biofilm on coated scaffolds by collagen/SeNPs was significantly reduced compared to the uncoated ones. © The Author(s) 2022. |
| Databáze: | OpenAIRE |
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