Glycerokinase in human adipose tissue
| Autor: | Rosemary L. Ryall, R. B. Goldrick |
|---|---|
| Rok vydání: | 1977 |
| Předmět: |
Adult
Glycerol Male medicine.medical_specialty Clinical chemistry Adipose tissue Biology Biochemistry chemistry.chemical_compound Adenosine Triphosphate Glycerol Kinase Internal medicine medicine Humans Aged Skin chemistry.chemical_classification Body Weight Phosphotransferases Organic Chemistry Temperature Cell Biology Hydrogen-Ion Concentration Middle Aged Kinetics Enzyme Endocrinology Adipose Tissue chemistry Female Subcutaneous adipose tissue Lineweaver–Burk plot Omentum Adenosine triphosphate |
| Zdroj: | Lipids. 12:272-277 |
| ISSN: | 1558-9307 0024-4201 |
| Popis: | The presence of glycerokinase has been demonstrated in human omental and subcutaneous adipose tissue. The enzyme reaction showed a linear time course for 5 min at 30 C and pH optima at pH 7.6 and 9.0. Saturation of the enzyme was observed at 1.8 mM adenosine triphosphate (ATP) and the double reciprocal plot of activity vs. ATP concentration was nonlinear giving two apparent Km values of 0.094 and 0.518 mM. The apparent Km for glycerol, 0.112 mM, was obtained from a linear double reciprocal plot, and the enzyme was saturated at about 0.4 mM glycerol. The activity of glycerokinase in human adipose tissue excised under general anaesthesia was low and was unrelated to adipose cell size or the degree of obesity of the subject from whom the fat was obtained. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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