Quantification of oxygen-induced retinopathy in the mouse: a model of vessel loss, vessel regrowth and pathological angiogenesis
| Autor: | Nathan M. Krah, Karen I. Guerin, Kip M. Connor, C. M. Aderman, Keirnan L. Willett, Lois E.H. Smith, Andreas Stahl, Roberta J. Dennison, Przemyslaw Sapieha, Jing Chen |
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| Rok vydání: | 2009 |
| Předmět: |
Pathology
medicine.medical_specialty Angiogenesis Biology Bioinformatics Article Retina General Biochemistry Genetics and Molecular Biology Neovascularization Mice chemistry.chemical_compound Retinal Diseases medicine Animals Regeneration Hyperoxia Neovascularization Pathologic Dissection Retinal Vessels Retinal Retinopathy of prematurity Diabetic retinopathy medicine.disease eye diseases Oxygen Disease Models Animal medicine.anatomical_structure chemistry sense organs medicine.symptom Retinopathy |
| Zdroj: | Nature Protocols. 4:1565-1573 |
| ISSN: | 1750-2799 1754-2189 |
| Popis: | The mouse model of oxygen-induced retinopathy (OIR) has been widely used in studies related to retinopathy of prematurity, proliferative diabetic retinopathy and in studies evaluating the efficacy of antiangiogenic compounds. In this model, 7-d-old (P7) mouse pups with nursing mothers are subjected to hyperoxia (75% oxygen) for 5 d, which inhibits retinal vessel growth and causes significant vessel loss. On P12, mice are returned to room air and the hypoxic avascular retina triggers both normal vessel regrowth and retinal neovascularization (NV), which is maximal at P17. Neovascularization spontaneously regresses between P17 and P25. Although the OIR model has been the cornerstone of studies investigating proliferative retinopathies, there is currently no harmonized protocol to assess aspects of angiogenesis and treatment outcome. In this protocol we describe standards for mouse size, sample size, retinal preparation, quantification of vascular loss, vascular regrowth, NV and neovascular regression. |
| Databáze: | OpenAIRE |
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