IgG antibodies against human papillomavirus type 16 E7 proteins in cervicovaginal washing fluid from patients with cervical neoplasia

Autor: Theo A. Out, S. P. Tjiong-A-Hung, Matthé P.M. Burger, J. Ter Schegget, Linda Struyk, Ming Y. Tjiong, N. van der Vange
Přispěvatelé: Obstetrics and gynaecology, Amsterdam Reproduction & Development (AR&D), Other departments
Rok vydání: 2000
Předmět:
Zdroj: International Journal of Gynecological Cancer, 10(4), 296-304. Wolters Kluwer Health
Tjiong, M Y, Ter Schegget, J, Tjiong-A-Hung, S P, Out, T A, Van Der Vange, N, Burger, M P M & Struyk, L 2000, ' IgG antibodies against human papillomavirus type 16 E7 proteins in cervicovaginal washing fluid from patients with cervical neoplasia ', International Journal of Gynecological Cancer, vol. 10, no. 4, pp. 296-304 . https://doi.org/10.1046/j.1525-1438.2000.010004296.x
International journal of gynecological cancer, 10(4), 296-304. Wolters Kluwer Health
ISSN: 1525-1438
1048-891X
DOI: 10.1046/j.1525-1438.2000.010004296.x
Popis: Little information is available about the cervicovaginal mucosal antibodies against human papillomavirus (HPV) proteins. In this study specific IgG antibodies against HPV 16 E7 protein were determined in paired samples of cervicovaginal washing fluid and serum from patients with cervical cancer (n = 22), cervical intraepithelial neoplasia (CIN) (n = 38), healthy individuals (n = 22), and serum from children (n = 41) by a radioactive immunoprecipitation assay (RIPA). HPV 16 E7 specific IgG antibodies were found in cervicovaginal washings (n = 8) and in sera (n = 8) of the patients with cervical cancer. About 60% of the patients with HPV 16 positive cervical cancer had HPV 16 E7 specific IgG antibodies. Titration studies showed that the IgG antibody reactivity in cervicovaginal washings was higher than in the paired serum samples of six patients with cervical cancer (P < 0.001). In the CIN group we found no IgG reactivity in the serum, but in five patients we found a low IgG reactivity in the cervicovaginal washings. No IgG reactivity was found in cervicovaginal washings and sera from healthy individuals and sera from children. HPV 16 E7 specific IgG antibodies seem to be locally produced in a number of patients with HPV 16 positive (pre)malignant cervical lesions. For more definitive evidence for the local production of these antibodies immunostaining should be performed to demonstrate the presence of specific anti-HPV 16 E7 IgG producing plasma cells in the cervical epithelium.
Databáze: OpenAIRE
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