Human apurinic/apyrimidinic endonuclease 1 (APE1) has 3' RNA phosphatase and 3' exoribonuclease activities
| Autor: | Chow H. Lee, Manbir Chohan, Sebastian Mackedenski, Wai-Ming Li |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
DNA Repair
RNA-dependent RNA polymerase Biology Hydroxylamines Substrate Specificity Structural Biology Exoribonuclease Endoribonucleases Benzoquinones DNA-(Apurinic or Apyrimidinic Site) Lyase Humans Molecular Biology Flavonoids RNA Nuclease protection assay Non-coding RNA Molecular biology Phosphoric Monoester Hydrolases DNA/RNA non-specific endonuclease Biochemistry RNA editing Exoribonucleases Propionates Exoribonuclease activity Half-Life |
| Zdroj: | Journal of molecular biology. 427(2) |
| ISSN: | 1089-8638 |
| Popis: | Apurinic/apyrimidinic endonuclease 1 (APE1) is the predominant mammalian enzyme in DNA base excision repair pathway that cleaves the DNA backbone immediately 5' to abasic sites. In addition to its abasic endonuclease activity, APE1 has 3' phosphatase and 3'-5' exonuclease activities against DNA. We recently identified APE1 as an endoribonuclease that preferentially cleaves at UA, UG, and CA sites in single-stranded regions of RNAs and can regulate c-myc mRNA level and half-life in cells. APE1 can also endonucleolytically cleave abasic single-stranded RNA. Here, we show for the first time that the human APE1 has 3' RNA phosphatase and 3' exoribonuclease activities. Using three distinct RNA substrates, we show that APE1, but not RNase A, can remove the phosphoryl group from the 3' end of RNA decay products. Studies using various site-directed APE1 mutant proteins (H309N, H309S, D283N, N68A, D210N, Y171F, D308A, F266A, and D70A) suggest that the 3' RNA phosphatase activity shares the same active center as its other known nuclease activities. A number of APE1 variants previously identified in the human population, including the most common D148E variant, have greater than 80% reduction in the 3' RNA phosphatase activity. APE1 can remove a ribonucleotide from the 3' overhang of RNA decay product, but its 3'-5' exoribonuclease activity against unstructured poly(A), poly(C), and poly(U) RNAs is relatively weak. This study further underscores the significance of understanding the role of APE1 in RNA metabolism in vivo. |
| Databáze: | OpenAIRE |
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