A human colon tumour antigen associated with β2-Microglobulin and isolated from solid tumour, serum and urine, is unrelated to carcinoembryonic antigen
| Autor: | D.M.P. Thomson, Donna N. Tataryn, Josie C. Weatherhead, Paula Friedlander, Joyce Rauch, Rosemarie Schwartz, Phil Gold, Joseph Shuster |
|---|---|
| Rok vydání: | 1980 |
| Předmět: |
Pathology
medicine.medical_specialty Colorectal cancer Epitope Epitopes Carcinoembryonic antigen Affinity chromatography Antigens Neoplasm medicine Humans Antiserum biology Beta-2 microglobulin business.industry Leukocyte Adherence Inhibition Test Cancer Radioimmunoassay General Medicine medicine.disease digestive system diseases Carcinoembryonic Antigen Colonic Neoplasms Cancer research biology.protein Electrophoresis Polyacrylamide Gel Lipoproteins HDL beta 2-Microglobulin business |
| Zdroj: | European Journal of Cancer (1965). 16:539-551 |
| ISSN: | 0014-2964 |
| Popis: | The antigen-induced leukocyte adherence inhibition response is directed to an organ-type specific neoantigen. Cancers of the colorectum, pancreas and stomach each expressed a unique organ-specific neoantigen. The relationship of the colon tumour antigen to the previously described carcinoembryonic antigen (CEA) of GIT cancers was examined by a tube blocking LAI assay and CEA radioimmunoassay. The colon tumour antigen was papain-solubilized from colon cancer membranes. An affinity column of horse anti-human β 2 -microglobulin bound the colon tumour antigen but did not bind CEA. In contrast, the colon tumour antigen failed to bind to an affinity column of antisera prepared to cell surface proteins that had failed to bind to the anti-human β 2 -microglobulin affinity column. Both the colon tumour antigen and CEA existed in the serum of patients with metastatic cancer. The colon tumour antigen co-isolated with the HDL fraction of serum by polyanion precipitation which suggested that the colon tumour antigen was lipoprotein in composition. By contrast, CEA was recovered in the non-lipoprotein fraction of serum. A proportion of the colon tumour antigen and CEA in the serum were eliminated into the urinary protein by filtration in the kidney. The results of the present study indicate that the colon tumour antigen epitope and the CEA epitope exist on separate molecules. |
| Databáze: | OpenAIRE |
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