A mouse juvenile or adult slice with preserved functional nigro-striatal dopaminergic neurons
| Autor: | Hervé Fiorentino, François Gonon, Constance Hammond, C. Lopez, Rachida Ammari |
|---|---|
| Přispěvatelé: | Institut de Neurobiologie de la Méditerranée [Aix-Marseille Université] (INMED - INSERM U1249), Aix Marseille Université (AMU)-Institut National de la Santé et de la Recherche Médicale (INSERM), Laboratoire Mouvement Adaptation Cognition (MAC), Université Bordeaux Segalen - Bordeaux 2-Centre National de la Recherche Scientifique (CNRS), Tyzio, Roman |
| Rok vydání: | 2009 |
| Předmět: |
Nomifensine
Patch-Clamp Techniques MESH: Dopamine Uptake Inhibitors Dopamine MESH: Neurons Striatum MESH: Animals Newborn MESH: Corpus Striatum Membrane Potentials Mice 0302 clinical medicine Dopamine Uptake Inhibitors Neural Pathways Basal ganglia Electrochemistry MESH: Animals MESH: Tyrosine 3-Monooxygenase Neurons 0303 health sciences General Neuroscience Dopaminergic Age Factors MESH: Electric Stimulation Substantia Nigra Subthalamic nucleus medicine.drug MESH: Axons Tyrosine 3-Monooxygenase MESH: Substantia Nigra Biophysics Substantia nigra MESH: Dopamine [SDV.BC]Life Sciences [q-bio]/Cellular Biology In Vitro Techniques Biology MESH: Dendrites 03 medical and health sciences Slice preparation MESH: Mice Inbred C57BL MESH: Patch-Clamp Techniques medicine MESH: Membrane Potentials Animals [SDV.BC] Life Sciences [q-bio]/Cellular Biology MESH: Mice MESH: Biophysics 030304 developmental biology MESH: Age Factors MESH: Electrochemistry Pars compacta MESH: Neural Pathways Dendrites Axons Corpus Striatum Electric Stimulation Mice Inbred C57BL Animals Newborn nervous system MESH: Nomifensine Neuroscience 030217 neurology & neurosurgery |
| Zdroj: | Neuroscience Neuroscience, Elsevier-International Brain Research Organization, 2009, 159 (1), pp.3-6. ⟨10.1016/j.neuroscience.2008.10.051⟩ Neuroscience, 2009, 159 (1), pp.3-6. ⟨10.1016/j.neuroscience.2008.10.051⟩ |
| ISSN: | 0306-4522 1873-7544 |
| DOI: | 10.1016/j.neuroscience.2008.10.051 |
| Popis: | International audience; The effect of endogenous dopamine on the activity of target neurons recorded with patch clamp or Ca2+ imaging techniques in slices has been studied to date with intra-striatal stimuli. Yet, this approach is severely handicapped by the nonphysiological and nonspecific stimulation of local neurons and fibers within the striatum. We now report a new juvenile and adult mouse slice preparation in which a component of the nigro-striatal dopaminergic pathway is preserved in its entirety, from cell bodies to axon terminals. This tilted parasagittal slice (380-400 microm) just medial to the subthalamic nucleus contains functional nigro-striatal neurons as assessed by morphological examination of tyrosine hydroxylase positive cell bodies and axons, combined with electrochemical assays of dopamine release in the striatum in response to stimulation of the substantia nigra pars compacta. The nigro-striatal slice constitutes a suitable in vitro preparation to determine the impact of endogenously released dopamine on target neurons of the striatum. |
| Databáze: | OpenAIRE |
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