Basophil allergen threshold sensitivity and component-resolved diagnostics improve hazelnut allergy diagnosis
| Autor: | Magnus P. Borres, S. G. O. Johansson, Caroline Nilsson, Josef Brandström, Gunnar Lilja, A. C Sundqvist, Anna Nopp |
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| Rok vydání: | 2014 |
| Předmět: |
Male
Adolescent Oral food challenge business.industry Immunology Basophil Allergens Immunoglobulin E medicine.disease_cause Basophils medicine.anatomical_structure Allergen Corylus Double-Blind Method Hazelnut allergy medicine Immunology and Allergy Humans Clinical significance Female Nut Hypersensitivity business Child Sensitization |
| Zdroj: | Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology. 45(9) |
| ISSN: | 1365-2222 |
| Popis: | IgE sensitization to hazelnut is common, especially in birch endemic areas. However, its clinical significance often needs to be confirmed by a food challenge.To evaluate the clinical significance of IgE antibodies to hazelnut components and basophil allergen threshold sensitivity (CD-sens) to hazelnut, in relation to double-blind placebo-controlled food challenge (DBPCFC) in children with a suspected hazelnut allergy.Forty children underwent a DBPCFC. CD-sens to hazelnut as well as IgE antibodies to hazelnut and its components Cor a 1, Cor a 8, Cor a 9 and Cor a 14 were analysed. Serum tryptase was measured before, during and after DBPCFC.Eight children had a positive DBPCFC, and all of them had a high CD-sens value to hazelnut. Of the 32 children that passed the DBPCFC, 31 were very low or negative in CD-sens. A positive DBPCFC corresponded with significantly higher CD-sens values (median 8.9, range 3.3-281) compared to children negative in challenge (median 0.05, range 0-34.7, P0.0001). Children positive in challenge also had higher levels of IgE-ab to Cor a 9 and Cor a 14 (P0.01 and P0.001, respectively) compared with those with a negative challenge. In relation to the results from DBPCFC, the sensitivity of CD-sens and IgE-ab to Cor a 14 was excellent (100%) and the specificity was very high (97% and94%, respectively). Five of the eight patients positive at challenge showed an increase in tryptase20% compared to tryptase baseline levels.CD-sens and component-resolved diagnostics to hazelnut, used separately or in combination, may improve the diagnostic accuracy and safety and reduce overdiagnosis of hazelnut allergy. |
| Databáze: | OpenAIRE |
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