Sleep Loss Drives Brain Region-Specific and Cell Type-Specific Alterations in Ribosome-Associated Transcripts Involved in Synaptic Plasticity and Cellular Timekeeping
Autor: | James Delorme, Marcus Donnelly, Sha Jiang, Sara J. Aton, Lijing Wang, Donald Popke, Carlos Puentes-Mestril |
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Rok vydání: | 2021 |
Předmět: |
Male
0301 basic medicine Interneuron Development/Plasticity/Repair translation Hippocampus Neocortex interneuron Biology Inhibitory postsynaptic potential Mice 03 medical and health sciences 0302 clinical medicine Interneurons medicine Animals principal neurons Research Articles Neuronal Plasticity Pyramidal Cells General Neuroscience sleep deprivation Sleep deprivation 030104 developmental biology medicine.anatomical_structure nervous system circadian rhythms Protein Biosynthesis plasticity Forebrain Synaptic plasticity Excitatory postsynaptic potential medicine.symptom Ribosomes Neuroscience 030217 neurology & neurosurgery |
Zdroj: | The Journal of Neuroscience |
ISSN: | 1529-2401 0270-6474 |
Popis: | Sleep and sleep loss are thought to impact synaptic plasticity, and recent studies have shown that sleep and sleep deprivation (SD) differentially affect gene transcription and protein translation in the mammalian forebrain. However, much less is known regarding how sleep and SD affect these processes in different microcircuit elements within the hippocampus and neocortex, for example, in inhibitory versus excitatory neurons. Here, we use translating ribosome affinity purification (TRAP) and in situ hybridization to characterize the effects of sleep versus SD on abundance of ribosome-associated transcripts in Camk2a-expressing (Camk2a+) pyramidal neurons and parvalbumin-expressing (PV+) interneurons in the hippocampus and neocortex of male mice. We find that while both Camk2a+ neurons and PV+ interneurons in neocortex show concurrent SD-driven increases in ribosome-associated transcripts for activity-regulated effectors of plasticity and transcriptional regulation, these transcripts are minimally affected by SD in hippocampus. Similarly, we find that while SD alters several ribosome-associated transcripts involved in cellular timekeeping in neocortical Camk2a+ and PV+ neurons, effects on circadian clock transcripts in hippocampus are minimal, and restricted to Camk2a+ neurons. Taken together, our results indicate that SD effects on transcripts associated with translating ribosomes are both cell type-specific and brain region-specific, and that these effects are substantially more pronounced in the neocortex than the hippocampus. We conclude that SD-driven alterations in the strength of synapses, excitatory-inhibitory (E-I) balance, and cellular timekeeping are likely more heterogeneous than previously appreciated. SIGNIFICANCE STATEMENT Sleep loss-driven changes in transcript and protein abundance have been used as a means to better understand the function of sleep for the brain. Here, we use translating ribosome affinity purification (TRAP) to characterize changes in abundance of ribosome-associated transcripts in excitatory and inhibitory neurons in mouse hippocampus and neocortex after a brief period of sleep or sleep loss. We show that these changes are not uniform, but are generally more pronounced in excitatory neurons than inhibitory neurons, and more pronounced in neocortex than in hippocampus. |
Databáze: | OpenAIRE |
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