Evaluation of different measles IgG assays based on recombinant proteins using a panel of low-titre sera
| Autor: | Fabienne B. Bouche, R.L. de Swart, Claude P. Muller, Helma W. Vos, Francois Schneider, A.D.M.E. Osterhaus, H.K Hartter, F Hanses |
|---|---|
| Přispěvatelé: | Virology |
| Rok vydání: | 2000 |
| Předmět: |
Hemagglutination
Paramyxoviridae Enzyme-Linked Immunosorbent Assay Antibodies Viral Sensitivity and Specificity Measles Measles virus SDG 3 - Good Health and Well-being Morbillivirus Neutralization Tests Virology medicine Humans Mononegavirales Hemagglutination assay biology Hemagglutination Tests Reference Standards Flow Cytometry biology.organism_classification medicine.disease Recombinant Proteins Hemagglutinins ROC Curve Evaluation Studies as Topic Immunoglobulin G Immunology biology.protein Antibody |
| Zdroj: | Journal of Virological Methods, 84, 191-200. Elsevier |
| ISSN: | 0166-0934 |
| Popis: | During the WHO campaign to eradicate measles, accurate discrimination between immune and non-immune individuals will become increasingly important. Due to waning immunity in vaccinated populations, the performance of a measles IgG assay depends mainly on its ability to detect reliably seronegative individuals among many vaccinees with low antibody levels. New serological tests based on recombinant proteins detect only a fraction of the total measles virus (MV) specific antibodies. Therefore, several assays based on recombinant MV-haemagglutinin (ELISA and flow cytometry) or MV-fusion protein (flow cytometry) as well as neutralisatlon and haemagglutination test have been evaluated using a large panel of low-titre and negative sera. Since such an evaluation is highly dependent on threshold values for positivity, the receiver operating characteristic curve analysis was applied. The H-FACS and the H-ELISA showed the best performing characteristics (specificity: 97.4 and 96.1%, respectively; sensitivity: 88.1 and 89.6%, respectively) and may be an alternative to the neutralisation assay. The number of undefined/grey zone sera was significantly lower compared to a commercial whole virus-based ELISA and therefore fewer individuals would be vaccinated unnecessarily. |
| Databáze: | OpenAIRE |
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