Rechargeable anti-microbial adhesive formulation containing cetylpyridinium chloride montmorillonite

Autor: Hideki Obika, Bart Van Meerbeek, Kenya Matsuo, Akihiro Matsukawa, Takumi Okihara, Kumiko Yoshihara, Yoji Makita, Yasuhiro Yoshida, Noriyuki Nagaoka
Rok vydání: 2019
Předmět:
02 engineering and technology
Cetylpyridinium chloride
Biochemistry
Streptococcus mutans
chemistry.chemical_compound
Mice
Antiseptic
Anti-Infective Agents
X-Ray Diffraction
Antibacterial agent
education.field_of_study
biology
Cell Death
General Medicine
021001 nanoscience & nanotechnology
Antimicrobial
Recharge
Bentonite
Powders
0210 nano-technology
S. mutans
Biotechnology
medicine.drug_class
0206 medical engineering
Population
Adhesive
Biomedical Engineering
Cetylpyridinium chloride (CPC)
Cetylpyridinium
macromolecular substances
Microbial Sensitivity Tests
Cell Line
Biomaterials
Adhesives
medicine
Animals
education
Molecular Biology
Montmorillonite
technology
industry
and agriculture
biology.organism_classification
020601 biomedical engineering
Antibacterial
Drug Liberation
chemistry
Dentin
Nuclear chemistry
Zdroj: Acta biomaterialia. 100
ISSN: 1878-7568
Popis: Long-term anti-bacterial effect is a desired ability of any dental material in combating tooth caries as one of the most common and widespread persistent diseases today. Among several cationic quaternary ammonium compounds with antiseptic properties, cetylpyridinium chloride (CPC) is often used in mouthrinses and toothpastes. In this study, we incorporated CPC in a soft phyllosilicate mineral (clay), referred to as montmorillonite (Mont), to enable gradual CPC release with rechargeability. Besides measuring CPC release and recharge, we examined the anti-bacterial effect, cytotoxicity and bonding effectiveness of five experimental adhesive formulations, prepared by adding 1 and 3 wt% CPC_Mont, 3 wt% Mont (without CPC), and 1 and 3 wt% CPC (without Mont) to the commercial adhesive Clearfil S3 Bond ND Quick ('C-S3B'; Kuraray Noritake). Strong inhibition of Streptococcus mutans biofilm formation by CPC_Mont adhesives was confirmed by optical density and SEM. CPC release from CPC_Mont adhesives was higher and lasted longer than from CPC adhesives, while CPC_Mont adhesives could also be recharged with CPC upon immersion in 2 wt% CPC. In conclusion, CPC_Mont technology rendered adhesives anti-bacterial properties with recharge ability, this without reducing its bonding potential, neither increasing its cytotoxicity. STATEMENT OF SIGNIFICANCE: Dental caries is one of the most prevalent chronic diseases in the population worldwide and is the major cause of tooth loss. In this study, we developed cetylpyridinium chloride (CPC) loaded montmorillonite (CPC-Mont) with a long-term antibacterial efficacy to prevent caries. CPC is an antibacterial agent approved by FDA, used as an OTC drug and contained in oral hygiene aids. CPC-Mont was incorporated in a dental adhesive to gradually release CPC. CPC_Mont technology rendered adhesives anti-bacterial properties with rechargeability, this without reducing its bonding potential, neither increasing its cytotoxicity. ispartof: ACTA BIOMATERIALIA vol:100 pages:388-397 ispartof: location:England status: published
Databáze: OpenAIRE
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