Influence of embryonic conditioned media upon the endometrial β-adrenergic receptor
| Autor: | DA Benitez, ME Bruzzone, F Zegers-Hochschild, C Fabres, EA Castellón |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
Adult
medicine.medical_specialty media_common.quotation_subject Biology Embryo Culture Techniques Endometrium Pregnancy Internal medicine Receptors Adrenergic beta Mole Gene expression medicine Humans Embryo Implantation Ovulation Cells Cultured Progesterone media_common Estradiol Obstetrics and Gynecology Embryo Embryo Mammalian Embryonic stem cell Endocrinology Reproductive Medicine Cell culture Culture Media Conditioned Female Human endometrium Developmental Biology Hormone |
| Zdroj: | Reproductive BioMedicine Online. 11:58-63 |
| ISSN: | 1472-6483 |
| DOI: | 10.1016/s1472-6483(10)61299-0 |
| Popis: | The main objective of this study was to evaluate the influence of the products secreted by the human embryo upon the three subtypes of beta-AR (beta1, beta2, beta3). Cell cultures were developed using endometrial biopsies, taken on day 7 after ovulation, from four healthy women35 years of age, with regular cycles and infertility due only to male factors. Embryos from women with a normal uterine cavity and endometrial lining were incubated until they reached the 4-cell stage, before being transferred to their mother's uterus. Culture media for embryo incubation were derived from two groups: (i) embryos that achieved pregnancy, (ii) embryos which failed to implant. Control and experimental endometrial cell culture plates were treated with the two embryo culture media, with or without 10(-9) mol/l oestradiol and 10(-7) mol/l progesterone for 48 h. Expression of the three subtypes of beta-AR was assessed by RT-PCR. Beta1-AR was expressed in both control and experimental plates; beta2-AR was expressed only in plates incubated with embryonic culture media of embryos which achieved pregnancy, in both hormonal conditions, with or without oestradiol and progesterone. Beta3-AR was not expressed in any condition. Thus secretory products of human embryos may influence gene expression of beta2-AR concentrations in the human endometrium, and this subtype of beta-AR may be involved in implantation. |
| Databáze: | OpenAIRE |
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