Structure of a peptidoglycan-related polysaccharide from Providencia alcalifaciens O45
| Autor: | Antoni Rozalski, Lei Wang, Lu Feng, A. S. Shashkov, Bin Liu, Nina A. Kocharova, Małgorzata Siwińska, Y. A. Knirel, Olga G. Ovchinnikova, Anna N. Kondakova |
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| Rok vydání: | 2012 |
| Předmět: |
Bacterial capsule
Magnetic Resonance Spectroscopy Lipopolysaccharide Molecular Sequence Data Peptidoglycan Providencia Biology Polysaccharide Biochemistry Bacterial cell structure Acetylglucosamine chemistry.chemical_compound Cell Wall Humans Bacterial Capsules chemistry.chemical_classification Alanine Bacterial polysaccharide O Antigens General Medicine Carbohydrate Sequence chemistry N-Acetylmuramic acid Muramic Acids Heteronuclear single quantum coherence spectroscopy |
| Zdroj: | Biochemistry (Moscow). 77:609-615 |
| ISSN: | 1608-3040 0006-2979 |
| Popis: | A polysaccharide was isolated from the opportunistic human pathogen Providencia alcalifaciens O45:H26 by extraction with aqueous phenol and studied by sugar and methylation analyses along with (1)H and (13)C NMR spectroscopy, including two-dimensional ROESY and H-detected (1)H,(13)C HSQC experiments. The polysaccharide contains N-acetylglucosamine and N-acetylmuramic acid (D-GlcpNAc3Rlac) amidated with L-alanine and has the following structure: →4)-β-D-GlcpNAc-(1→4)-β-D-GlcpNAc3(Rlac-L-Ala)-(1→. The polysaccharide possesses a remarkable structural similarity to the bacterial cell wall peptidoglycan. It is not unique to the strain studied but is common to strains of at least four P. alcalifaciens O-serogroups (O3, O24, O38, and O45). No evidence was obtained that the polysaccharide is associated with the LPS, and hence it might represent a bacterial capsule component. |
| Databáze: | OpenAIRE |
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