Inflammatory Cytokine-Producing Cells and Inflammation Markers in the Synovium of Osteoarthritis Patients Evidenced in Human Herpesvirus 7 Infection
| Autor: | Mihails Tarasovs, Sofija Semenistaja, Zaiga Nora-Krukle, Modra Murovska, Valerija Groma, Sandra Skuja, Simons Svirskis |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Male medicine.medical_treatment viruses Herpesvirus 7 Human lcsh:Chemistry 0302 clinical medicine Virus latency Medicine lcsh:QH301-705.5 Spectroscopy Aged 80 and over Synovitis Synovial Membrane virus diseases General Medicine Middle Aged Computer Science Applications Cytokine medicine.anatomical_structure PCR immunohistochemistry CD4 Antigens Cytokines Tumor necrosis factor alpha ELISA Female medicine.symptom Adult Roseolovirus Infections Inflammation synovium Article Catalysis Inorganic Chemistry 03 medical and health sciences HHV-7 Immune system Antigen Osteoarthritis Humans Physical and Theoretical Chemistry Molecular Biology Aged 030203 arthritis & rheumatology business.industry Interleukin-6 Tumor Necrosis Factor-alpha Organic Chemistry medicine.disease cytokines osteoarthritis 030104 developmental biology lcsh:Biology (General) lcsh:QD1-999 Immunology DNA Viral Synovial membrane business Biomarkers |
| Zdroj: | International Journal of Molecular Sciences Volume 21 Issue 17 International Journal of Molecular Sciences, Vol 21, Iss 6004, p 6004 (2020) |
| ISSN: | 1422-0067 |
| Popis: | A direct association between joint inflammation and the progression of osteoarthritis (OA) has been proposed, and synovitis is considered a powerful driver of the disease. Among infections implicated in the development of joint disease, human herpesvirus 7 (HHV-7) infection remains poorly characterized. Therefore, we assessed synovitis in OA patients determined the occurrence and distribution of the HHV-7 antigen within the synovial membrane of OA-affected subjects and correlated plasma levels of the pro-inflammatory cytokines tumor necrosis factor (TNF), interleukin-6 (IL-6), and TNF expressed locally within lesioned synovial tissues with HHV-7 observations, suggesting differences in persistent latent and active infection. Synovial HHV-7, CD4, CD68, and TNF antigens were detected immunohistochemically. The plasma levels of TNF and IL-6 were measured by an enzyme-linked immunosorbent assay. Our findings confirm the presence of persistent HHV-7 infection in 81.5% and reactivation in 20.5% of patients. In 35.2% of patients, virus-specific DNA was extracted from synovial membrane tissue samples. We evidenced the absence of histopathologically detectable synovitis and low-grade changes in the majority of OA patients enrolled in the study, in both HHV-7 PCR+ and HHV-7 PCR‒ groups. The number of synovial CD4-positive cells in the HHV-7 polymerase chain reaction (PCR)+ group was significantly higher than that in the HHV-7 PCR‒ group. CD4- and CD68-positive cells were differently distributed in both HHV-7 PCR+ and HHV-7 PCR‒ groups, as well as in latent and active HHV-7 infection. The number of TNF+ and HHV-7+ lymphocytes, as well as HHV-7+ vascular endothelial cells, was strongly correlated. Vascular endothelial cells, especially in the case of infection reactivation, appeared vulnerable. The balance between virus latency and reactivation is a long-term relationship between the host and infectious agent, and the immune system appears to be involved in displaying overreaction when a shift in the established equilibrium develops. |
| Databáze: | OpenAIRE |
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