Evaluation of supercritical carbon dioxide extraction to obtain bioactive compounds from Vernonia amygdalina Delile leaves
| Autor: | Terra Lemos Caroline Ortega, Edson Antonio da Silva, Marcos Rodrigo Traczynski, Erika Ohta Watanabe, Lúcio Cardozo-Filho, Hori Carla Eponina, Fabíola Medeiros da Costa, Sarah Arvelos |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
DPPH
020209 energy General Chemical Engineering antioxidant activity 02 engineering and technology vernonia amygdalina delile chemistry.chemical_compound 0202 electrical engineering electronic engineering information engineering lcsh:Chemical engineering supercritical extraction lcsh:HD9650-9663 0505 law Supercritical carbon dioxide Chromatography bioactive compounds biology Chemistry Vernonia amygdalina 05 social sciences Extraction (chemistry) Supercritical fluid extraction mathematical modeling phytochemical components lcsh:TP155-156 biology.organism_classification Supercritical fluid Yield (chemistry) 050501 criminology Gas chromatography lcsh:Chemical industries |
| Zdroj: | Chemical Industry and Chemical Engineering Quarterly, Vol 26, Iss 2, Pp 113-124 (2020) |
| ISSN: | 2217-7434 1451-9372 |
| Popis: | Literature reports have shown that supercritical extraction is a promising method to obtain bioactive compounds used to develop drugs based on natural products. This work investigated the yield, antioxidant activity, and phytochemical constituents of the extracts obtained from Vernonia amygdalina Delile leaves with supercritical CO2. The supercritical extraction was examined at temperatures of 40, 50 and 60?C and at pressures of 200 and 250 bar. The supercritical fluid extraction (SFE) method was compared with the classical Soxhlet using different solvents at atmospheric pressure. The overall yield obtained using SFE varied from 0.69 to 1.24%. The extraction conditions which favored the highest yield were 60?C and 250 bar. The overall SFE curves were fitted using three mathematical models. All of them were able to describe satisfactorily the extraction kinetics. Spline model presented the best fit to experimental data and was able to characterize constant and decreasing extraction rate periods. Extracts were characterized by gas chromatography coupled with mass spectrometry technique and some major components were identified. The antioxidant activity tests showed that the SFE extracts had low antioxidant activity exhibiting the estimated concentration of extract required to reduce 50% of the stable free radical DPPH (EC50) values to be between 622.62 and 937.88 ?g mL-1. |
| Databáze: | OpenAIRE |
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