Cyclin-Dependent Kinase CRK9, Required for Spliced Leader trans Splicing of Pre-mRNA in Trypanosomes, Functions in a Complex with a New L-Type Cyclin and a Kinetoplastid-Specific Protein
| Autor: | Sung Hee Park, Justin K. Kirkham, Arthur Günzl, Nitika Badjatia, Daniela Luz Ambrósio |
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| Přispěvatelé: | University of Connecticut Health Center, The Pennsylvania State University, Universidade de São Paulo (USP) |
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Polyadenylation RNA splicing Trans-splicing RNA polymerase II Protozoology Biochemistry Trans-Splicing Mice Gene expression RNA Precursors Cell Cycle and Cell Division Post-Translational Modification Phosphorylation lcsh:QH301-705.5 Phylogeny Protozoans Mice Inbred BALB C biology Cyclin-Dependent Kinases 3. Good health Nucleic acids Cell Processes Female RNA Polymerase II Sequence Analysis Research Article lcsh:Immunologic diseases. Allergy RNA Spliced Leader Trypanosoma Immunology Trypanosoma brucei brucei Research and Analysis Methods Microbiology 03 medical and health sciences Cyclin-dependent kinase Sequence Motif Analysis Virology Cyclins Proliferating Cell Nuclear Antigen Genetics Animals Molecular Biology Techniques Sequencing Techniques Gene Molecular Biology 030102 biochemistry & molecular biology Organisms RNA Biology and Life Sciences Proteins Kinetoplastids Cell Biology Molecular biology Parasitic Protozoans 030104 developmental biology lcsh:Biology (General) RNA processing biology.protein Parasitology lcsh:RC581-607 Sequence Alignment Trypanosoma Brucei Gambiense |
| Zdroj: | Scopus Repositório Institucional da UNESP Universidade Estadual Paulista (UNESP) instacron:UNESP PLoS Pathogens PLoS Pathogens, Vol 12, Iss 3, p e1005498 (2016) |
| Popis: | In eukaryotes, cyclin-dependent kinases (CDKs) control the cell cycle and critical steps in gene expression. The lethal parasite Trypanosoma brucei, member of the phylogenetic order Kinetoplastida, possesses eleven CDKs which, due to high sequence divergence, were generically termed CDC2-related kinases (CRKs). While several CRKs have been implied in the cell cycle, CRK9 was the first trypanosome CDK shown to control the unusual mode of gene expression found in kinetoplastids. In these organisms, protein-coding genes are arranged in tandem arrays which are transcribed polycistronically. Individual mRNAs are processed from precursor RNA by spliced leader (SL) trans splicing and polyadenylation. CRK9 ablation was lethal in cultured trypanosomes, causing a block of trans splicing before the first transesterification step. Additionally, CRK9 silencing led to dephosphorylation of RNA polymerase II and to hypomethylation of the SL cap structure. Here, we tandem affinity-purified CRK9 and, among potential CRK9 substrates and modifying enzymes, discovered an unusual tripartite complex comprising CRK9, a new L-type cyclin (CYC12) and a protein, termed CRK9-associated protein (CRK9AP), that is only conserved among kinetoplastids. Silencing of either CYC12 or CRK9AP reproduced the effects of depleting CRK9, identifying these proteins as functional partners of CRK9 in vivo. While mammalian cyclin L binds to CDK11, the CRK9 complex deviates substantially from that of CDK11, requiring CRK9AP for efficient CRK9 complex formation and autophosphorylation in vitro. Interference with this unusual CDK rescued mice from lethal trypanosome infections, validating CRK9 as a potential chemotherapeutic target. Author Summary Kinetoplastids are vector-borne, unicellular parasites that cause devastating human diseases in the tropics and subtropics of the world. Interestingly, kinetoplastids express protein-coding genes differently than other eukaryotes by producing polycistronic pre-mRNAs that require processing into individual mRNAs by spliced leader trans splicing and polyadenylation. While each parasite mRNA is trans-spliced, this particular splicing process is absent in mammalian and insect hosts of kinetoplastids. We recently discovered that the cyclin-dependent kinase (CDK) CRK9 of the kinetoplastid Trypanosoma brucei is essential for trans splicing and parasite viability. Due to the essential roles of CDKs in cell proliferation, CDK inhibition is an established strategy against cancer, suggesting that chemotherapeutic intervention of CRK9 will halt proliferation of kinetoplastid parasites in their hosts. To enable inhibitor studies of CRK9, we have characterized the CRK9 enzyme complex and discovered a new cyclin and a kinetoplastid-specific protein, both of which are essential for the formation of active CRK9. The tripartite nature of the CRK9 complex and sequence insertions that disrupt both kinase and cyclin domains suggest that CRK9 deviates structurally from human CDKs. Finally, by demonstrating that CRK9 ablation prevented trypanosomes from establishing lethal infections in mice, we validated CRK9 as a potential anti-parasitic drug target. |
| Databáze: | OpenAIRE |
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