Prevalence of TEM, SHV, and CTX-M Beta-Lactamase genes in the urinary isolates of a tertiary care hospital
| Autor: | Ganesh S Bhatambare, Meena Varma, Trupti Bajpai, Maneesha Pandey |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
medicine.drug_class polymerase chain reaction medicine.medical_treatment Urinary system 030106 microbiology Antibiotics Cephalosporin extended-spectrum beta-lactamases law.invention Microbiology 03 medical and health sciences law polycyclic compounds medicine Monobactams Gene Polymerase chain reaction biology business.industry biochemical phenomena metabolism and nutrition bacterial infections and mycoses biology.organism_classification Enterobacteriaceae Beta-lactam antibiotics 030104 developmental biology Beta-lactamase bacteria Medicine Original Article business |
| Zdroj: | Avicenna Journal of Medicine Avicenna Journal of Medicine, Vol 07, Iss 01, Pp 12-16 (2017) |
| ISSN: | 2249-4464 2231-0770 |
| DOI: | 10.4103/2231-0770.197508 |
| Popis: | Introduction: Extended-spectrum beta-lactamases (ESBLs) are the major cause of resistance to beta-lactam antibiotics such as penicillins, cephalosporins, and monobactams. They are derived from the narrow-spectrum beta-lactamases (TEM-1, TEM-2, or SHV-1) by mutations that alter the amino acid configuration around the enzyme active site. Aim: To determine the prevalence of ESBL (bla TEM , bla CTX-M , and bla SHV ) genes among the members of Enterobacteriaceae. Methodology: The present prospective study was carried out from January 2015 to June 2015 in the Department of Microbiology and Molecular Medicine of a Teaching Tertiary Care Hospital. A total of 526 urine samples were studied. Seventy-eight isolates were subjected to polymerase chain reaction for detection of ESBL genes. Results: In our study, ESBL genes were detected among 18 (45%) phenotypically confirmed ESBL producers and 20 (52.5%) phenotypically confirmed non-ESBL producers. The gene that predominated was bla TEM (48.7%), followed by bla CTX-M (7.6%) and bla SHV (5.1%). Conclusion: Definitive identification of ESBL genes is only possible by molecular detection methods. Phenotypic tests need to be evaluated periodically as their performance may change with the introduction of new enzymes. |
| Databáze: | OpenAIRE |
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