Functional roles of ST8SIA3-mediated sialylation of striatal dopamine D2 and adenosine A2A receptors
| Autor: | Hsing-Lin Lai, Yijuang Chern, Kay-Hooi Khoo, Hui-Mei Chen, Chung-Yi Wu, Ken Kitajima, Jian-Jing Siew, Chihiro Sato, Shih-Chieh Tsai, Chien-Yu Lin, Cheng-Te Hsiao, Kuo-Shiang Liao, Hua-Chien Chang |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Receptor Adenosine A2A Population Heteromer Adenosine A2A receptor Striatum Molecular neuroscience Article lcsh:RC321-571 03 medical and health sciences Cellular and Molecular Neuroscience Mice 0302 clinical medicine Membrane Microdomains Dopamine Dopamine receptor D2 medicine Animals Receptor education lcsh:Neurosciences. Biological psychiatry. Neuropsychiatry Biological Psychiatry G protein-coupled receptor Mice Knockout Neurons education.field_of_study Chemistry Receptors Dopamine D2 Corpus Striatum Sialyltransferases Cell biology Psychiatry and Mental health 030104 developmental biology Pharmacogenetics 030217 neurology & neurosurgery medicine.drug |
| Zdroj: | Translational Psychiatry Translational Psychiatry, Vol 9, Iss 1, Pp 1-13 (2019) |
| ISSN: | 2158-3188 |
| Popis: | Sialic acids are typically added to the end of glycoconjugates by sialyltransferases. Among the six ST8 α-N-acetyl-neuraminide α-2,8-sialyltransferases (ST8SIA) existing in adult brains, ST8SIA2 is a schizophrenia-associated gene. However, the in vivo substrates and physiological functions of most sialyltransferases are currently unknown. The ST8SIA3 is enriched in the striatum. Here, we showed that ablation of St8sia3 in mice (St8sia3-KO) led to fewer disialylated and trisialylated terminal glycotopes in the striatum of St8sia3-KO mice. Moreover, the apparent sizes of several striatum-enriched G-protein-coupled receptors (GPCRs) (including the adenosine A2A receptor (A2AR) and dopamine D1/D2 receptors (D1R and D2R)) were smaller in St8sia3-KO mice than in WT mice. A sialidase treatment removed the differences in the sizes of these molecules between St8sia3-KO and WT mice, confirming the involvement of sialylation. Expression of ST8SIA3 in the striatum of St8sia3-KO mice using adeno-associated viruses normalized the sizes of these proteins, demonstrating a direct role of ST8SIA3. The lack of ST8SIA3-mediated sialylation altered the distribution of these proteins in lipid rafts and the interaction between D2R and A2AR. Locomotor activity assays revealed altered pharmacological responses of St8sia3-KO mice to drugs targeting these receptors and verified that a greater population of D2R formed heteromers with A2AR in the striatum of St8sia3-KO mice. Since the A2AR-D2R heteromer is an important drug target for several basal ganglia diseases (such as schizophrenia and Parkinson’s disease), the present study not only reveals a crucial role for ST8SIA3 in striatal functions but also provides a new drug target for basal ganglia-related diseases. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|