Sub-milliMorgan map of the proximal part of mouse Chromosome 17 including the hybrid sterility 1 gene
| Autor: | M. Loudová, Vladimir Vincek, Gregorová S, J. Čapková, M. Mňuková-Fajdelová, Jiri Forejt, Hans Lehrach, M. Hoglund, Zdenek Trachtulec, Jan Klein, Renata M.J. Hamvas |
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| Rok vydání: | 1996 |
| Předmět: |
Genetic Markers
Male Candidate gene Genotype Molecular Sequence Data Biology Genome Mice Genetics Animals Gene Infertility Male Polymorphism Single-Stranded Conformational DNA Primers Recombination Genetic Mice Inbred C3H Base Sequence H-2 Antigens Chromosome Chromosome Mapping Molecular biology Chromosome 17 (human) Mice Inbred C57BL Genetic marker Multigene Family Microsatellite Hybridization Genetic Female Restriction fragment length polymorphism Polymorphism Restriction Fragment Length |
| Zdroj: | Mammalian genome : official journal of the International Mammalian Genome Society. 7(2) |
| ISSN: | 0938-8990 |
| Popis: | We have generated a high-resolution genetic map, 0.071 cM per backcross animal, of the 13 cM T-H2 region of the mouse Chromosome (Chr) 17. The map contains two phenotypic loci, T and Hst1, 12 RFLP markers, and 24 microsatellite loci. The Hst1 gene was mapped to a chromosomal interval contained within a single 580-kb YAC clone. The FFEH11 YAC is 0.44 cM long and carries, besides the Hst1 gene, five polymorphic DNA markers and recombination breakpoints of six backcross animals. Two candidate genes for Hst1 were identified based on their location and testicular expression. These are Tbp and D17Ph4e. The submilliMorgan map of the T-H2 region revealed significant clustering of (CA)n loci. The clustering, if shown to be a common feature in the mouse genome, may cause gaps in the physical map of the mouse genome. |
| Databáze: | OpenAIRE |
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