Insulin antibodies in insulin-dependent diabetics before insulin treatment
| Autor: | Kenneth R. Lyen, Olga Tatpati, Prima Raghu, Thomas L. Paquette, Peter Clemons, Christopher M. Asplin, Jerry P. Palmer |
|---|---|
| Rok vydání: | 1983 |
| Předmět: |
Adult
medicine.medical_specialty Adolescent medicine.medical_treatment Insulin Antibody Immune system Diabetes mellitus Internal medicine medicine Humans Insulin Child Autoantibodies Multidisciplinary biology business.industry Gamma globulin medicine.disease Endocrinology Diabetes Mellitus Type 1 biology.protein Radiobinding assay Antibody business Insulin dependent |
| Zdroj: | Science (New York, N.Y.). 222(4630) |
| ISSN: | 0036-8075 |
| Popis: | A sensitive assay was used to measure the binding of iodine-125-labeled insulin in serum obtained from 112 newly diagnosed insulin-dependent diabetics before insulin treatment was initiated. Two groups of nondiabetics served as controls: children with a variety of diseases other than diabetes and nondiabetic siblings of insulin-dependent diabetics. Eighteen of the diabetics were found to have elevated binding and 36 were above the 95th percentile of control values. The insulin-binding protein is precipitated by antibody to human immunoglobulin G, has a displacement curve that is parallel and over the same concentration range as serum from long-standing insulin-dependent diabetics, and elutes from a Sephacryl S-300 column at the position of gamma globulin. These insulin antibodies are present in a large percentage of newly diagnosed, untreated diabetics and may be an immune marker of B-cell damage. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|