An Investigation Into the Bias Between Liquid Chromatography–Tandem Mass Spectrometry and an Enzyme Multiplied Immunoassay Technique for the Measurement of Mycophenolic Acid
| Autor: | Paul J. Taylor, Michael E. Franklin, J. Michael Tredger, Christopher E. Gonde, Eyrun N Einarsdottir, Nigel W. Brown, Maria Pires |
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| Rok vydání: | 2010 |
| Předmět: |
Adult
Male Quality Control Analyte Population Kidney Function Tests Tandem mass spectrometry Mycophenolic acid Bias Tandem Mass Spectrometry Liquid chromatography–mass spectrometry Enzyme Multiplied Immunoassay Technique medicine Humans Drug Interactions Pharmacology (medical) education Chromatography High Pressure Liquid Pharmacology Enzyme multiplied immunoassay technique education.field_of_study Chromatography medicine.diagnostic_test Chemistry Mycophenolic Acid Immunoassay Linear Models Female Drug Monitoring Quantitative analysis (chemistry) Immunosuppressive Agents medicine.drug |
| Zdroj: | Therapeutic Drug Monitoring. 32:420-426 |
| ISSN: | 0163-4356 |
| DOI: | 10.1097/ftd.0b013e3181e6b348 |
| Popis: | Mycophenolic acid is now the second most widely used immunosuppressant in solid organ transplantation. Overestimation of mycophenolic acid concentration is a recognized problem of immunoassay, and high-performance liquid chromatography with ultraviolet detection methods have long analysis times and a risk of analyte coelution which may compromise high sample throughput in a clinically meaningful time frame. A novel liquid chromatography-tandem mass spectrometry assay for mycophenolic acid was developed using very small (10 microL) sample volumes and evaluated in comparison with an established immunological assay. The enzyme mediated immunoassay showed a median positive bias compared with liquid chromatography-tandem mass spectrometry of 14.6%. Linear regression analysis showed a significant positive impact of bilirubin (r2 = 0.230) on bias with further increases of r2 to 0.261, 0.286, and 0.294 with the stepwise addition of creatinine, hematocrit, and gamma-glutamyl transpeptidase, respectively. The impact of comedication and transplant type depended on the patient population: analysis of all samples showed opposing effects to analysis of those samples lacking data with biochemical variables above. The liquid chromatography-tandem mass spectrometry method described in this report is capable of measuring mycophenolic acid concentrations in very small sample volumes and in a timely fashion without the significant overestimates characterizing enzyme mediated immunoassay measurements in patients with serologic features characterizing liver or renal graft rejection. |
| Databáze: | OpenAIRE |
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