Induction of experimental autoimmune thyroiditis (EAT) by heat-denaturated thyroglobulin (Tg)
| Autor: | Karine Mignon-Godefroy, M. P. Brazillet, Jeannine Charreire |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
Protein Denaturation
Hot Temperature Endocrinology Diabetes and Metabolism medicine.medical_treatment Biology Autoantigens Thyroglobulin Thyroiditis Autoimmune thyroiditis Mice Endocrinology Antigen Internal Medicine medicine Animals Cytotoxic T cell B cell Autoantibodies Macrophages Thyroid Thyroiditis Autoimmune Autoantibody General Medicine medicine.disease Coculture Techniques medicine.anatomical_structure Immunology Mice Inbred CBA Female Immunization Lymph Nodes T-Lymphocytes Cytotoxic |
| Zdroj: | Experimental and Clinical Endocrinology & Diabetes. 104:23-25 |
| ISSN: | 1439-3646 0947-7349 |
| DOI: | 10.1055/s-0029-1211675 |
| Popis: | Experimental Autoimmune Thyroiditis (EAT) is characterized by autoreactive T and B cell responses, assessed by a marked lymphocytic infiltration of the thyroid gland by T cells and the occurrence of circulating autoantibodies (AAb) to thyroglobulin (Tg). It was recently reported that administration of denaturated exogenous antigens primes class I-restricted cytotoxic T cells in vivo. Since cytotoxic T cells are involved in EAT development, porcine Tg (pTg) was heat-denaturated, i.v. injected into CBA/J mice and features of EAT evaluated. Simultaneously, mice were immunized with pTg and adjuvants and evaluation of EAT performed. We found that heat-denaturated pTg (hdpTg) induced EAT in recipient mice similar to native pTg/adjuvants. Surprisingly, whereas Tg-specific cytotoxic T cells were regularly found in lymph node cells from hdpTg or native pTg immunized mice, proliferative responses were only detected using T cells from native pTg immunized mice. Autoantibodies to pTg were decreased by a factor 30 in sera from mice immunized with hdpTg. These data further emphasized the role of Tg-specific cytotoxic T cells in EAT. |
| Databáze: | OpenAIRE |
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