Genome-Wide Analysis and Expression Tendency of Banana (Musa acuminata L.) Calcineurin B-Like (MaCBL) Genes under Potassium Stress
| Autor: | Yan Zhao, Ying Xiong, Fenlian Tang, Yuan Yao, Yingdui He, Xuejun Lin, Ruimei Li, Xiaomin Yin, Jianchun Guo, Yongxia Liu, Yangjiao Zhou, Jiao Liu, Wang Lixia |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0106 biological sciences
CBL yeast mutant Transgene Mutant subcellular location Sequence alignment Plant Science lcsh:Plant culture Horticulture Biology 01 natural sciences Genome 03 medical and health sciences potassium stress Musa acuminata Gene expression lcsh:SB1-1110 Gene 030304 developmental biology Genetics 0303 health sciences biology.organism_classification Elongation factor banana gene expression functional complementation 010606 plant biology & botany |
| Zdroj: | Horticulturae Volume 7 Issue 4 Horticulturae, Vol 7, Iss 70, p 70 (2021) |
| ISSN: | 2311-7524 |
| DOI: | 10.3390/horticulturae7040070 |
| Popis: | Calcineurin B-like (CBL) proteins are reported to play significant roles in plant development and ion-transport regulation. Potassium shortages are a serious problem in banana cultivation. However, to date, the members of the banana CBL gene family, and their function in regulating potassium stress, remain unclear. In this study, 11 CBL genes were identified from the banana genome and grouped into four groups (Group I–IV) based on their phylogenetic relationships. The genomic features of these MaCBL genes were analyzed, focusing on their gene structures, standpat motifs, chromosomal distributions, and evolutionary history. Expression pattern analysis revealed that the MaCBLs were function-specific. Further qRT-PCR analysis indicated that the presence of MaCBL2 was indeed a response to potassium deficiency stress. The MaCBL2 gene was cloned, and sequence alignment indicated that it contained four elongation factor hand (EF-hand) domains, the conserved N-terminal myristoylation domain “MGCXXS/K(T)” and the “FPSF” motif. Subcellular location analysis showed that MaCBL2 was located in the plasma membrane, nucleus and cytoplasm. The overexpression of MaCBL2 could restore the growth of the yeast mutant R5421 on a K+-deficient medium. The overexpression of MaCBL2 could promote the root length of transgenic seedlings on K+-deficient medium. These findings indicate that MaCBL2 was, in our study, the key gene of the CBL family in responding to potassium deficiency in bananas. Our discoveries have established a considerable basis for the further study and application of MaCBL genes. |
| Databáze: | OpenAIRE |
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