Quantification of 17 Endogenous and Exogenous Steroidal Hormones in Equine and Bovine Blood for Doping Control with UHPLC-MS/MS
| Autor: | Gianni Sagratini, Fulvio Laus, Stefano Sartori, Manuela Cortese, Giovanni Caprioli, Riccardo Petrelli, Sauro Vittori, Michele Genangeli, Ahmed M. Mustafa, Massimo Ricciutelli |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Calibration curve
medicine.medical_treatment Pharmaceutical Science Endogeny 01 natural sciences Article Steroid Pharmacy and materia medica Drug Discovery medicine Androstenedione equine blood Testosterone Detection limit Chromatography HPLC-MS/MS 010405 organic chemistry Chemistry 010401 analytical chemistry anti-doping bovine blood 0104 chemical sciences Triple quadrupole mass spectrometer RS1-441 steroidal hormones Medicine Molecular Medicine Hormone |
| Zdroj: | Pharmaceuticals, Vol 14, Iss 393, p 393 (2021) Pharmaceuticals Volume 14 Issue 5 |
| Popis: | A simple and fast analytical method able to simultaneously identify and quantify 17 endogenous and exogenous steroidal hormones was developed in bovine and equine blood using UHPLC-MS/MS. A total amount of 500 µL of sample was deproteinized with 500 µL of a mixture of methanol and zinc sulfate and evaporated. The mixture was reconstituted with 50 µL of a solution of 25% methanol and injected in the UHPLC-MS/MS triple quadrupole. The correlation coefficients of the calibration curves of the analyzed compounds were in the range of 0.9932–0.9999, and the limits of detection and quantification were in the range of 0.023–1.833 and 0.069–5.5 ppb, respectively. The developed method showed a high sensitivity and qualitative aspects allowing the detection and quantification of all steroids in equine and bovine blood. Moreover, the detection limit of testosterone (50 ppt) is half of the threshold admitted in plasma (100 ppt). Once validated, the method was used to quantify 17 steroid hormones in both bovine and equine blood samples. The primary endogenous compounds detected were corticosterone (range 0.28–0.60 ppb) and cortisol (range 0.44–10.00 ppb), followed by androstenedione, testosterone and 11-deoxycortisol. |
| Databáze: | OpenAIRE |
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