Sequence-independent VIDISCA-454 technique to discover new viruses in canine livers
| Autor: | van der Heijden, M., de Vries, M., van Steenbeek, F.G., Favier, R.P., Deijs, M., Brinkhof, B., Rothuizen, J., van der Hoek, L., Penning, L.C., Advances in Veterinary Medicine, Tissue Repair, Geneeskunde van gezelschapsdieren |
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| Přispěvatelé: | Advances in Veterinary Medicine, Tissue Repair, Geneeskunde van gezelschapsdieren, Other departments, AII - Amsterdam institute for Infection and Immunity, Medical Microbiology and Infection Prevention |
| Rok vydání: | 2012 |
| Předmět: |
Virus Cultivation
040301 veterinary sciences Sequence analysis viruses Biology Adenoviruses Canine Virus Cell Line 0403 veterinary science 03 medical and health sciences Dogs Liver tissue Virology medicine Animals Viral etiology 030304 developmental biology Hepatitis 0303 health sciences 04 agricultural and veterinary sciences Sequence Analysis DNA medicine.disease 3. Good health Hepatitis Infectious Canine Liver Molecular Diagnostic Techniques Cell culture Viral hepatitis Nucleic Acid Amplification Techniques |
| Zdroj: | Journal of Virological Methods, 185(1), 152. Elsevier Journal of Virological Methods Journal of Virological Methods; Vol 185 Journal of virological methods, 185(1), 152-155. Elsevier |
| ISSN: | 0166-0934 |
| Popis: | In many mammals, viruses cause hepatitis. Despite many efforts a specific virus responsible for canine idiopathic hepatitis has not been identified. The discovery of a viral etiology in canine hepatitis will promote the development of specific drugs and vaccines for the treatment of idiopathic hepatitis in dogs. The objective of this study was the application of the sequence-independent Virus Discovery cDNA-amplified fragment length polymorphism (VIDISCA) technique combined with high through-put sequencing on a Roche-454 sequencer to identify unknown viruses. Liver tissue of a dog with idiopathic acute hepatitis was cultured on a canine liver cell line and the cell culture medium was submitted to the VIDISCA-454 technique. Without prior knowledge of the viral species involved, this technique identified Canine adenoyirus type 1 (CAV-1) as the infecting agent. This demonstrates the power of VIDISCA-454 to identify viruses, independent of preliminary information about the genomic sequence. Consequently, the strategy of propagation in this cell line followed by the VIDISCA-454 technique is valuable to identify the viral etiology of idiopathic hepatitis in dogs. (C) 2012 Elsevier B.V. All rights reserved |
| Databáze: | OpenAIRE |
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