Enzymatic production of bioactive docosahexaenoic acid phenolic ester

Autor: Thierry Oster, Francoise Chretien, Cédric Paris, Brenda N. Targino, Vanessa Cristina de Castro, Layal Dahdou, Paulo Henrique Alves da Silva, Stéphane Desobry, Catherine Humeau, Rosa-Maria Guéant, Mohamed H.H. Roby, Marion Huguet, Ahmad Allouche
Přispěvatelé: Laboratoire d'Ingénierie des Biomolécules (LIBio), Université de Lorraine (UL), Unité de Recherches Animal et Fonctionnalités des Produits Animaux (URAFPA), Institut National de la Recherche Agronomique (INRA)-Université de Lorraine (UL), Structure et Réactivité des Systèmes Moléculaires Complexes (SRSMC), Université de Lorraine (UL)-Centre National de la Recherche Scientifique (CNRS), Institut de Chimie du CNRS (INC)-Université de Lorraine (UL)-Centre National de la Recherche Scientifique (CNRS), French Ministry of Research and Higher Education, Lorraine Region, European Commission for Erasmus Mundus Grant, University of Damascus (Syria), CAPES/MEC-Brazilian Ministry of Education, Université de Lorraine (UL)-Institut National de la Recherche Agronomique (INRA)
Rok vydání: 2014
Předmět:
Male
Antioxidant
Erythrocytes
gallate egcg derivative
030309 nutrition & dietetics
medicine.medical_treatment
antioxidant activity
Vanillyl alcohol
polyunsaturated fatty-acid
Analytical Chemistry
Acylation
Ingredient
chemistry.chemical_compound
Mice
lipase-catalyzed synthesis
learning-ability
alzheimers-disease
Cells
Cultured
Neurons
0303 health sciences
biology
Chemistry
food and beverages
Brain
Esters
General Medicine
Fish oil
Neuroprotection
DHA
Neuroprotective Agents
Biochemistry
Docosahexaenoic acid
lipids (amino acids
peptides
and proteins)
Oxidoreductases
fish-oil
Docosahexaenoic Acids
Cell Survival
plant phenolic
Fungal Proteins
03 medical and health sciences
Functional food
medicine
[CHIM]Chemical Sciences
Animals
Enzymatic esterification
Rats
Wistar
Benzyl Alcohols
030304 developmental biology
vanillyl alcohol
Lipase
biology.organism_classification
Enzymes
Immobilized
transesterification
Diet
Rats
Mice
Inbred C57BL
Candida antarctica
Food Science
Zdroj: LOCUS Repositório Institucional da UFV
Universidade Federal de Viçosa (UFV)
instacron:UFV
Food Chemistry
Food Chemistry, Elsevier, 2015, 171, pp.397-404. ⟨10.1016/j.foodchem.2014.09.028⟩
ISSN: 1873-7072
0308-8146
Popis: International audience; Docosahexaenoic acid (DHA) is increasingly considered for its health benefits. However, its use as functional food ingredient is still limited by its instability. In this work, we developed an efficient and solvent-free bioprocess for the synthesis of a phenolic ester of DHA. A fed-batch process catalyzed by Candida antarctica lipase B was optimised, leading to the production of 440 g/L vanillyl ester (DHA-VE). Structural characterisation of the purified product indicated acylation of the primary OH group of vanillyl alcohol. DHA-VE exhibited a high radical scavenging activity in acellular systems. In vivo experiments showed increased DHA levels in erythrocytes and brain tissues of mice fed DHA-VE-supplemented diet. Moreover, in vitro neuroprotective properties of DHA-VE were demonstrated in rat primary neurons exposed to amyloid-beta oligomers. In conclusion, DHA-VE synergized the main beneficial effects of two common natural biomolecules and therefore appears a promising functional ingredient for food applications.
Databáze: OpenAIRE
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