Midtrimester Fetal Herpes Simplex-2 Diagnosis by Serology, Culture and Quantitative Polymerase Chain Reaction
| Autor: | Maria-Magdalena Patru, William M. Curtin, Amy L Scheible, Marilyn A. Menegus, Robert A. Mooney, Leon A. Metlay, Nancy L. Stanwood, C. Jeanne Peterson, Angela Dorgan |
|---|---|
| Rok vydání: | 2012 |
| Předmět: |
Adult
Embryology Pathology medicine.medical_specialty Amniotic fluid Herpesvirus 2 Human viruses Prenatal diagnosis Antibodies Viral Serology Young Adult Patient Education as Topic Pregnancy Prenatal Diagnosis Humans Medicine Radiology Nuclear Medicine and imaging Fetus medicine.diagnostic_test business.industry Viral culture Obstetrics and Gynecology Herpes Simplex General Medicine Amniotic Fluid Molecular Typing Real-time polymerase chain reaction Pregnancy Trimester Second Pediatrics Perinatology and Child Health Immunology Amniocentesis Echogenic Bowel Female alpha-Fetoproteins business Abortion Eugenic |
| Zdroj: | Fetal Diagnosis and Therapy. 33:133-136 |
| ISSN: | 1421-9964 1015-3837 |
| Popis: | The acquisition of herpes simplex virus (HSV) in utero comprises a minority of neonatal herpes infections. Prenatal diagnosis is rare. We describe a midtrimester diagnosis of fetal HSV-2 infection. Ultrasound at 20 weeks for elevated maternal serum α-fetoprotein (MSAFP) showed lagging fetal growth, echogenic bowel, echogenic myocardium, and liver with a mottled pattern of echogenicity. Amniocentesis demonstrated normal karyotype, elevated AFP and positive acetylcholinesterase. Culture isolated HSV-2 with an aberrant growth pattern. Maternal serology was positive for HSV-2. Quantitative DNA polymerase chain reaction (PCR) showed 59 million copies/ml. Fetal autopsy demonstrated widespread tissue necrosis but only sparse HSV-2 inclusions. Fetal HSV-2 infection can be suspected when an elevated MSAFP accompanies ultrasound findings suggesting perinatal infection. Maternal HSV serology, amniotic fluid culture and quantitative PCR are recommended for diagnostic certainty and counseling. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|