Inactivation of anoctamin-6/Tmem16f, a regulator of phosphatidylserine scrambling in osteoblasts, leads to decreased mineral deposition in skeletal tissues
| Autor: | Uwe Kornak, Stefanie Gross, Markus Moser, Bent Brachvogel, Hans-Markus Munter, Manuela Wuelling, Milana Chinenkova, Andrea Vortkamp, Yvonne Krause, Harald W. A. Ehlen |
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| Rok vydání: | 2012 |
| Předmět: |
musculoskeletal diseases
Endocrinology Diabetes and Metabolism Anoctamins Phosphatidylserines Chondrocyte Bone and Bones Uncalcified osteoid Extracellular matrix chemistry.chemical_compound Mice Calcification Physiologic Phospholipid scrambling Annexin medicine Animals Orthopedics and Sports Medicine Phospholipid Transfer Proteins Cells Cultured Periosteum Osteoblasts Chemistry Skull Osteoblast Biological Transport Phosphatidylserine Embryo Mammalian Mice Mutant Strains Cell biology medicine.anatomical_structure Phenotype Biochemistry Animals Newborn Biologie |
| Zdroj: | Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research. 28(2) |
| ISSN: | 1523-4681 |
| Popis: | During vertebrate skeletal development, osteoblasts produce a mineralized bone matrix by deposition of hydroxyapatite crystals in the extracellular matrix. Anoctamin6/Tmem16F (Ano6) belongs to a conserved family of transmembrane proteins with chloride channel properties. In addition, Ano6 has been linked to phosphatidylserine (PS) scrambling in the plasma membrane. During skeletogenesis, Ano6 mRNA is expressed in differentiating and mature osteoblasts. Deletion of Ano6 in mice results in reduced skeleton size and skeletal deformities. Molecular analysis revealed that chondrocyte and osteoblast differentiation are not disturbed. However, mutant mice display increased regions of nonmineralized, Ibsp-expressing osteoblasts in the periosteum during embryonic development and increased areas of uncalcified osteoid postnatally. In primary Ano6−/− osteoblasts, mineralization is delayed, indicating a cell autonomous function of Ano6. Furthermore, we demonstrate that calcium-dependent PS scrambling is impaired in osteoblasts. Our study is the first to our knowledge to reveal the requirement of Ano6 in PS scrambling in osteoblasts, supporting a function of PS exposure in the deposition of hydroxyapatite. © 2013 American Society for Bone and Mineral Research |
| Databáze: | OpenAIRE |
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