Inonotus obliquus polysaccharide ameliorates dextran sulphate sodium induced colitis involving modulation of Th1/Th2 and Th17/Treg balance
| Autor: | Yao Xiao, Yi Fang Chen, Fan Ping Meng, Jin Juan Zheng, Gui Hua Jin, Hong Hua Li, Dan Jin, Fangfang Li, Chao Qu, Quan Xin Jin |
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| Rok vydání: | 2021 |
| Předmět: |
Male
Colon Biomedical Engineering Pharmaceutical Science Medicine (miscellaneous) Spleen 02 engineering and technology Pharmacology Occludin Inflammatory bowel disease T-Lymphocytes Regulatory 03 medical and health sciences 0302 clinical medicine Western blot Oral administration medicine Mesenteric lymph nodes Animals Lymphocyte Count Colitis Th1-Th2 Balance Mice Inbred BALB C biology medicine.diagnostic_test Chemistry Basidiomycota Dextran Sulfate Fungal Polysaccharides General Medicine 021001 nanoscience & nanotechnology medicine.disease biology.organism_classification Inflammatory Bowel Diseases Disease Models Animal STAT Transcription Factors medicine.anatomical_structure 030220 oncology & carcinogenesis Inonotus obliquus Cytokines Th17 Cells 0210 nano-technology Biotechnology Signal Transduction |
| DOI: | 10.6084/m9.figshare.7828370.v11 |
| Popis: | Inflammatory bowel disease (IBD) is an intestinal chronic inflammatory disease, and is related to imbalance of CD4+T subsets. However, the current treatments of chronic colitis are not ideal and have potential side effects. Therefore, more effective and safer biologically active substances which are extracted from natural plants have been widely concerned. In this study, it was found that Inonotus obliquus polysaccharides (IOP), the main bioactive constituent of Inonotus obliquus, can alleviate dextran sodium sulfate-induced chronic murine intestinal inflammation. Oral administration of IOP (100, 200, 300 mg/kg) can significantly reduce the disease active index and alleviate the pathological changes in colitis mice, where the tight junction proteins Occludin and ZO-1 losses in colon tissues were reduced. It can also regulate imbalanced Th1/Th2 and Th17/Treg in colon tissues, mesenteric lymph nodes and spleen using Reverse Transcription-Polymerase Chain Reaction detection and flow cytometry. Immunohistochemistry and western blot assays further revealed the modulatory effect of IOP on the p-STAT1, p-STAT6, p-STAT3 expression, which promoted the balance of Th1/Th2, Th17/Treg in the colon of chronic colitis mice. In short, these results indicated that IOP was potentially effective therapeutic agent for IBD. |
| Databáze: | OpenAIRE |
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