Characterization and function analysis of Epinephelus coioides Hsp40 response to Vibrio alginolyticus and SGIV infection
| Autor: | Xiaohong Huang, Yun Yang, Yan Ou, Pin-Hong Li, Jingguang Wei, He-Jia Chen, Xiao-Hong Xin, Qiwei Qin, Youhua Huang, Hongyan Sun |
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| Rok vydání: | 2021 |
| Předmět: |
Fish Proteins
Vibrio alginolyticus biology Activator (genetics) Ranavirus General Medicine HSP40 Heat-Shock Proteins Aquatic Science Epinephelus biology.organism_classification Southeast asian DNA Virus Infections Microbiology Iridovirus Fish Diseases Open reading frame Viral replication Heat shock protein Animals Environmental Chemistry Bass Grouper Phylogeny |
| Zdroj: | Fish & Shellfish Immunology. 118:396-404 |
| ISSN: | 1050-4648 |
| DOI: | 10.1016/j.fsi.2021.09.030 |
| Popis: | Heat shock protein 40 (Hsp40), a member of Heat shock proteins (Hsps) family, plays a crucial role in regulation of cell proliferation, survival and apoptosis in mammals. In this study, Hsp40, EcHsp40, was identified from Epinephelus coioides, an economically important marine-cultured fish in China and Southeast Asian counties. The full length of EcHsp40 was 2236 bp in length containing a 1026 bp open reading frame (ORF) encoding 341 amino acids, with a molecular mass of 37.88 kDa and a theoretical pI of 9.09. EcHsp40 has two conserved domains DnaJ and DnaJ_C. EcHsp40 mRNA was detected in all tissues examined, and the expression was significantly up-regulated response to challenged with Vibrio alginolyticus or Singapore grouper iridovirus (SGIV), one of the important pathogens of marine fish. EcHsp40 was distributed in both the cytoplasm and nucleus, over-expression of EcHsp40 can inhibit the activity of nuclear factor-κB (NF-κB) and activator protein-1 (AP-1), significantly promote SGIV-induced apoptosis, intracellular caspase-3 activity and viral replication, suggesting that the EcHsp40 may play an important role in pathogenic stimulation. |
| Databáze: | OpenAIRE |
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