An automatic technique for the routine fractionation of the urinary hydroxyproline containing peptides
| Autor: | J.P. Borel, A. Garbe, A. Randoux, P.M. Joubert, A. Szymanovicz |
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| Rok vydání: | 1980 |
| Předmět: |
Adult
Bone disease Adolescent Urinary system Clinical Biochemistry Statistics as Topic Fraction (chemistry) Bone Neoplasms Fractionation Alkaline hydrolysis (body disposal) Chemical Fractionation Biochemistry Hydroxyproline chemistry.chemical_compound Pregnancy medicine Humans Statistical analysis Child Chromatography Autoanalysis Hydrolysis Biochemistry (medical) Bone metastasis General Medicine Middle Aged medicine.disease chemistry Female Peptides |
| Zdroj: | Clinica chimica acta; international journal of clinical chemistry. 100(2) |
| ISSN: | 0009-8981 |
| Popis: | A fully automatic method permits fractionation in a Biogel P2 column of the hydroxyproline-containing peptides into two fractions. The alkaline hydrolysis and the colorimetric evaluation of the liberated hydroxyproline are also completely automatic and allow calculation of the percentages of the two fractions. The first one, termed F1 fraction, contains the peptides of molecular weight larger than 1500, while the other, termed F2, contains the smaller peptides. The method was used for 223 assays. The F1 fraction is decreased in cases of Paget's bone disease. It is increased in cases of metastatic cancer of bone. Statistical analysis of the data demonstrates that this technique greatly improves the certainty of diagnosis when coupled to the assay of total hydroxyproline. When both total urinary hydroxyproline and the F1 fraction percentage are increased over threshold values of 485 μmol per 24 h and 28.4% respectively, the probability of the presence of a bone metastasis is 100%. |
| Databáze: | OpenAIRE |
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