Diamond Nanoparticles-Porphyrin mTHPP Conjugate as Photosensitizing Platform: Cytotoxicity and Antibacterial Activity
| Autor: | Verônica Ribeiro dos Santos, Oluwatobi S. Oluwafemi, Dayane Batista Tada, Ncediwe Tsolekile, Katia Conceição, Rafaela Campos Queiroz, Carolina R. Hurtado, Gabriela Ramos Hurtado, Milton Faria Diniz, Vladimir Jesus Trava-Airoldi, Mauricio S. Baptista, Gabrielle Lupeti de Cena, Alexandre Vieira Silva |
|---|---|
| Přispěvatelé: | Federal Institute of São Paulo (IFSP), Universidade de São Paulo (USP), Universidade Estadual Paulista (Unesp), Helmholtz Zentrum München, Technological Institute of Aeronautics (ITA), National Institute for Space Research (INPE), University of Johannesburg |
| Rok vydání: | 2021 |
| Předmět: |
General Chemical Engineering
Nanoparticle photosensitizers (PS) Photosensitizers (PS) Bacterial growth Article chemistry.chemical_compound drug-resistance Zeta potential General Materials Science Cytotoxicity QD1-999 photodynamic antimicrobial therapy (aPDT) Drug-resistance Photodynamic antimicrobial therapy (aPDT) Chemistry Porphyrin RESISTÊNCIA MICROBIANA ÀS DROGAS Nanoconjugate Photodynamic Antimicrobial Therapy (apdt) Photosensitizers (ps) Covalent bond nanoconjugate Antibacterial activity Nuclear chemistry Conjugate |
| Zdroj: | Nanomaterials Nanomaterials 11:1393 (2021) Nanomaterials, Vol 11, Iss 1393, p 1393 (2021) Volume 11 Issue 6 Scopus Repositório Institucional da UNESP Universidade Estadual Paulista (UNESP) instacron:UNESP Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP |
| ISSN: | 2079-4991 |
| Popis: | Conjugation of photosensitizers (PS) with nanoparticles has been largely used as a strategy to stabilize PS in the biological medium resulting in photosensitizing nanoparticles of enhanced photoactivity. Herein, (Meso-5, 10, 15, 20-tetrakis (3-hydroxyphenyl) phorphyryn (mTHPP) was conjugated with diamond nanoparticles (ND) by covalent bond. Nanoconjugate ND-mTHPP showed suitable stability in aqueous suspension with 58 nm of hydrodynamic diameter and Zeta potential of −23 mV. The antibacterial activity of ND-mTHPP was evaluated against Escherichia coli for different incubation times (0–24 h). The optimal activity was observed after 2 h of incubation and irradiation (660 nm 51 J/cm2) performed right after the addition of ND-mTHPP (100 μg/mL) to the bacterial suspension. The inhibitory activity was 56% whereas ampicillin at the same conditions provided only 14% of bacterial growth inhibition. SEM images showed agglomerate of ND-mTHPP adsorbed on the bacterial cell wall, suggesting that the antimicrobial activity of ND-mTHPP was afforded by inducing membrane damage. Cytotoxicity against murine embryonic fibroblast cells (MEF) was also evaluated and ND-mTHPP was shown to be noncytotoxic since viability of cells cultured for 24 h in the presence of the nanoconjugate (100 μg/mL) was 78%. Considering the enhanced antibacterial activity and the absence of cytotoxic effect, it is possible to consider the ND-mTHPP nanoconjugate as promising platform for application in antimicrobial photodynamic therapy (aPDT). |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|