Antigen presentation of the immunodominant T-cell epitope of the major mugwort pollen allergen, Art v 1, is associated with the expression of HLA-DRB1∗01
| Autor: | Fatima Ferreira, Beatrice Jahn-Schmid, Christof Ebner, Gabriele Gadermaier, Ingrid Fae, Azra Dedic, Barbara Bohle, Gottfried Fischer |
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| Rok vydání: | 2005 |
| Předmět: |
Adult
Male Adolescent Genotype T-Lymphocytes Immunology Epitopes T-Lymphocyte Immunodominance Human leukocyte antigen medicine.disease_cause Epitope Mugwort Allergen medicine Humans Immunology and Allergy Child HLA-DRB1 Plant Proteins Artemisia vulgaris Antigen Presentation biology Immunodominant Epitopes business.industry HLA-DR Antigens Allergens Antigens Plant Middle Aged biology.organism_classification Virology Case-Control Studies biology.protein Female Antibody business Cell Division HLA-DRB1 Chains |
| Zdroj: | Journal of Allergy and Clinical Immunology. 115:399-404 |
| ISSN: | 0091-6749 |
| DOI: | 10.1016/j.jaci.2004.10.010 |
| Popis: | Background Mugwort pollen allergens are the main cause of pollinosis in late summer in Europe. Ninety-five percent of patients allergic to mugwort are sensitized to the major allergen Art v 1. In contrast to other common pollen allergens that contain multiple T-cell epitopes, Art v 1 contains only 1 immunodominant T-cell epitope (Art v 1 25-36 ). Objective To characterize the minimal epitope of Art v 1 25-36 and to investigate a possible association of Art v 1 reactivity with HLA class II phenotypes. Methods Art v 1–specific T-cell lines and clones were established from 51 patients with clinically defined mugwort pollen allergy and IgE specific for Art v 1. To define minimal epitopes and binding sites within Art v 1 25-36 , truncated and single-substitution analog peptides were used for T-cell stimulation. To study HLA restriction, monoclonal anti-HLA antibodies and antigen-presenting cells with defined HLA-DRB and -DQB1 alleles were used. HLA typing of patients with allergy was performed by hybridization with sequence-specific oligonucleotides, PCR, and nucleotide sequencing. Results In 96% of the patients, a cellular response to Art v 1 25-36 was obtained, and a core region of 5 to 10 amino acids containing 3 to 5 amino acids essential for T-cell reactivity was defined. The frequency of HLA-DRB1 ∗ 01 in patients recognizing Art v 1 25-36 was significantly increased as compared with healthy controls (69% vs 21%; odds ratio, 8.45; P −6 ), and HLA-DRB1 ∗ 01 was identified as the main restriction element for the presentation of the immunodominant epitope. Conclusions Allergy to Art v 1 is characterized by a uniform T-cell response. The disease is apparently associated with the HLA-DR1 phenotype. Therefore, mugwort pollinosis is an ideal candidate for a peptide-based immunotherapy. |
| Databáze: | OpenAIRE |
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