Preliminary Evaluation of QuantiFERON SARS-CoV-2 and QIAreach Anti-SARS-CoV-2 Total Test in Recently Vaccinated Individuals
| Autor: | Nadia Pc. Allen, Jenny Howard, Sonia N. Rao, Davide Manissero, Vladyslav Nikolayevskyy, Soumya Jaganathan, Francis Stieber, Jeff Boyle |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Microbiology (medical)
biology business.industry SARS-CoV-2 T cell-mediated response T cell Brief Report Adaptive immunity Vaccination Interferon gamma release assay COVID-19 Acquired immune system QuantiFERON Infectious Diseases Immune system medicine.anatomical_structure Immunology biology.protein Cell-mediated immunity Medicine Antibody business CD8 |
| Zdroj: | Infectious Diseases and Therapy |
| ISSN: | 2193-6382 2193-8229 |
| Popis: | Introduction: There is an increasing body of evidence surrounding the importance of a T-cell mediated response to SARS-CoV-2 infection and after COVID-19 vaccination. In this internal feasibility study, we evaluated both the total antibody (IgA, IgM, and IgG) and T-cell responses in a cohort of COVID-19 convalescents and vaccinated individuals.Methods: Whole blood specimens were collected weekly from 12 subjects at different timepoints within/after the COVID-19 mRNA vaccination regimen and from 4 PCR-confirmed convalescent donors to measure durability of humoral and cell-mediated immune response. T-cell and Antibody responses were evaluated via the QuantiFERON SARS-CoV-2 Research Use Only (QFN SARS-CoV-2) assay which is an interferon gamma release assay (IGRA) and QIAreach Anti-SARS-CoV-2 Total (Anti-CoV-2) Test, respectively.Results: In a cohort of recently vaccinated individuals, subjects demonstrated robust total antibody and CD4+/CD8+ T-cell response to SARS-CoV-2 mRNA vaccines when followed for 2 months post 2nd dose. In most individuals, T-cell response declined between 1st and 2nd dose suggesting need for booster or completion of the 2-dose vaccine series. In a group of convalescent donors tested with QFN SARS-CoV-2 and Anti-CoV-2 tests, all patients had an antibody and T-cell response up to 1 year after natural infection.Conclusion: This small feasibility study demonstrates that QFN-SARS-CoV-2 test is able to identify CD4+ and CD8+ T-cell mediated responses in SARS-CoV-2 vaccinated subjects and those recovered from COVID-19, alongside a qualitative antibody response detectable via the QIAreach Anti-CoV2 Test. |
| Databáze: | OpenAIRE |
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