| Autor: |
Mari Merce Cascant-Vilaplana, Abel Albiach-Delgado, Marta Camprubí-Camprubí, Miriam Pérez-Cruz, Olga Gómez, Miguel Arráez, Marina López-Nogueroles, Julia Kuligowski, Máximo Vento |
| Rok vydání: |
2022 |
| Předmět: |
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| Zdroj: |
Free Radical Biology and Medicine. 179:164-169 |
| ISSN: |
0891-5849 |
| DOI: |
10.1016/j.freeradbiomed.2021.12.310 |
| Popis: |
Oxidative stress in the fetal period is associated with preterm birth as well as short and long-term adverse clinical outcomes. Here, an Ultra-Performance Liquid Chromatography-tandem Mass Spectrometry (UPLC-MS/MS) method for the simultaneous quantification of biomarkers of oxidative stress-derived damage to proteins and DNA in amniotic fluid (AF) samples is presented. Appropriate accuracy and precision levels, as well as sensitivity with limits of detection in the low nanomolar (2 nM) range were achieved. The analytical method was applied to a set of AF samples and reference ranges of the biomarker panel are presented. Median concentrations of biomarkers of protein oxidation (ortho-, 3-chloro-, and 3-nitrotyrosine) and their precursors (para-tyrosine and phenylalanine) ranged between 0.6 and 3 nM and 23 and 30 μM, respectively, while levels of a biomarker of DNA-oxidation (8-hydroxydeoxyguanosine, 8OHdG) and its precursor (2'-deoxyguanosine) were found to be 0.18 and 3 nM, respectively. Detection frequencies of all metabolites were 100% with exception of 3-chlorotyrosine (3Cl-Tyr) and 8OHdG, that were only detected in 8% of samples. The developed method may be applied in research studies focusing on oxidative stress-related complications during pregnancy. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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Full Text from ScienceDirect