The C. elegans mRNA decapping enzyme shapes morphology of cilia
| Autor: | Susumu Izumi, Takeshi Adachi, Keigo Nagahama |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Morphology (linguistics) Cell Biophysics Flagellum Biochemistry 03 medical and health sciences Endoribonucleases Organelle medicine Animals Cilia Caenorhabditis elegans Cell Shape Molecular Biology Neurons Messenger RNA biology Spermatozoon Cilium Cell Biology respiratory system biology.organism_classification Cell biology 030104 developmental biology medicine.anatomical_structure |
| Zdroj: | Biochemical and Biophysical Research Communications. 493:382-387 |
| ISSN: | 0006-291X |
| Popis: | Cilia and flagella are evolutionarily conserved organelles that protrude from cell surfaces. Most cilia and flagella are single rod-shaped but some cilia show a variety of shapes. For example, human airway epithelial cells are multiciliated, flagella of crayfish spermatozoon are star-like shaped, and fruit fly spermatozoon extends long flagella. In Caenorhabditis elegans, cilia display morphological diversity of shapes (single, dual rod-type and wing-like and highly-branched shapes). Here we show that DCAP-1 and DCAP-2, which are the homologues of mammalian DCP1 and DCP2 mRNA decapping enzymes, respectively, are involved in formation of dual rod-type and wing-like shaped cilia in C. elegans. mRNA decapping enzyme catalyzes hydrolysis of 5' cap structure of mRNA, which leads to degradation of mRNA. Rescue experiments showed that DCAP-2 acts not in glial cells surrounding cilia but in neurons. This is the first evidence to demonstrate that mRNA decapping is involved in ciliary shape formation. |
| Databáze: | OpenAIRE |
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