Expediting Comprehensive Molecular Analysis to Optimize Initial Treatment of Lung Cancer Patients With Minimal Smoking History
Autor: | Jochen K. Lennerz, Alice T. Shaw, Vashine Kamesan, Hayley Robinson, Ibiayi Dagogo-Jack, Mari Mino-Kenudson, A. John Iafrate, Anna F. Farago |
---|---|
Rok vydání: | 2019 |
Předmět: |
Adult
Male 0301 basic medicine Pulmonary and Respiratory Medicine Oncology medicine.medical_specialty Lung Neoplasms medicine.medical_treatment Smoking history DNA sequencing Targeted therapy 03 medical and health sciences 0302 clinical medicine Internal medicine Biopsy medicine Humans Initial treatment In patient Lung cancer Aged Aged 80 and over medicine.diagnostic_test business.industry Smoking High-Throughput Nucleotide Sequencing Middle Aged medicine.disease Molecular analysis 030104 developmental biology 030220 oncology & carcinogenesis Female business |
Zdroj: | Journal of Thoracic Oncology. 14:835-843 |
ISSN: | 1556-0864 |
Popis: | Lung cancer patients with tumors harboring actionable alterations can achieve very durable responses to first-line targeted therapy. However, identifying targetable alterations using next-generation sequencing (NGS) is a complex and time-intensive process. As actionable genetic alterations are enriched in lung cancers arising in patients with limited smoking history, we designed a workflow to expedite NGS testing for this group.We developed a protocol to allow for next-day extraction of nucleic acids from frozen tissue. Specimens were designated as high priority during sequencing. We determined the interval between biopsy and NGS results to evaluate whether the workflow reduced the pre-analytical period and in-laboratory turnaround time and allowed for rapid initiation of genotype-matched therapy.Between January 2017 and May 2018, 21 patients participated in the expedited sequencing program. The median interval between biopsy and NGS results was 10.7 days. Six patients received results within 1 week of biopsy. Performing molecular analysis on frozen tissue and prioritizing sequencing and analysis of these specimens reduced the pre-analytical period from 3.5 to 1.3 days (p0.0001) and shortened in-laboratory turnaround time by 3 days (11.8 versus 8.4 business days, p0.0001). Ninety-three percent of patients with an actionable molecular alteration received first-line targeted therapy. The median time-to-initiation of treatment was 19.7 days from biopsy.Sequencing and analyzing nucleic acids from frozen tissue is a practical strategy for shortening the time to matched therapy. The significant advantage of upfront treatment with targeted therapies in subsets of lung cancer patients provides rationale for developing workflows that accelerate comprehensive molecular analysis. |
Databáze: | OpenAIRE |
Externí odkaz: |