Light-sensing via hydrogen peroxide and a peroxiredoxin
| Autor: | Niek Welkenhuysen, Wolfgang Reiter, Aeid Igbaria, Mikael Molin, Michel B. Toledano, Friederike Roger, Mikael Käll, Gael Palais, Kristofer Bodvard, Natalie Romanov, Ken Peeters |
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| Přispěvatelé: | University of Gothenburg (GU), Chalmers University of Technology [Göteborg], Mass Spectrometry Facility, Max F. Perutz Laboratories, Service de Biologie Intégrative et Génétique Moléculaire (SBIGeM), Institut de Biologie Intégrative de la Cellule (I2BC), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS), Department of Biochemistry, Max F. Perutz Laboratories, University of Vienna, Dr Bohrgasse 9, A-1030 Vienna, Austria., ANR-12-BSV2-0012,ERRed,Compartimentation redox du GSH dans la cellule eukaryote et son role dans la regulation de l'homéostase redox du réticulum endoplasmique(2012) |
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Light Signal Transduction Saccharomyces cerevisiae Proteins Light Science General Physics and Astronomy Saccharomyces cerevisiae Biology Models Biological General Biochemistry Genetics and Molecular Biology Article 03 medical and health sciences Stress signalling medicine [CHIM]Chemical Sciences [SDV.BBM]Life Sciences [q-bio]/Biochemistry Molecular Biology Phosphorylation Protein kinase A Transcription factor Cell Nucleus Multidisciplinary General Chemistry Hydrogen Peroxide Peroxiredoxins Nutrient signalling Single-cell imaging Cyclic AMP-Dependent Protein Kinases 3. Good health Cell biology Protein Subunits Protein Transport 030104 developmental biology medicine.anatomical_structure Peroxidases Second messenger system Biocatalysis Thioredoxin Peroxiredoxin Nucleus Nuclear localization sequence |
| Zdroj: | Nature Communications Nature Communications, Nature Publishing Group, 2017, 8, pp.14791. ⟨10.1038/ncomms14791⟩ Nature Communications, 2017, 8, pp.14791. ⟨10.1038/ncomms14791⟩ Nature Communications, Vol 8, Iss 1, Pp 1-11 (2017) |
| ISSN: | 2041-1723 |
| Popis: | Yeast lacks dedicated photoreceptors; however, blue light still causes pronounced oscillations of the transcription factor Msn2 into and out of the nucleus. Here we show that this poorly understood phenomenon is initiated by a peroxisomal oxidase, which converts light into a hydrogen peroxide (H2O2) signal that is sensed by the peroxiredoxin Tsa1 and transduced to thioredoxin, to counteract PKA-dependent Msn2 phosphorylation. Upon H2O2, the nuclear retention of PKA catalytic subunits, which contributes to delayed Msn2 nuclear concentration, is antagonized in a Tsa1-dependent manner. Conversely, peroxiredoxin hyperoxidation interrupts the H2O2 signal and drives Msn2 oscillations by superimposing on PKA feedback regulation. Our data identify a mechanism by which light could be sensed in all cells lacking dedicated photoreceptors. In particular, the use of H2O2 as a second messenger in signalling is common to Msn2 oscillations and to light-induced entrainment of circadian rhythms and suggests conserved roles for peroxiredoxins in endogenous rhythms. While yeasts lack dedicated photoreceptors, they nonetheless possess metabolic rhythms responsive to light. Here the authors find that light signalling in budding yeast involves the production of H2O2, which in turn regulates protein kinase A through a peroxiredoxin-thioredoxin redox relay. |
| Databáze: | OpenAIRE |
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