In vitro immunization as an adjunct to the production of hybridomas producing antibodies against the lymphokine osteoclast activating factor
| Autor: | Richard A. Luben, Marjorie A. Mohler |
|---|---|
| Rok vydání: | 1980 |
| Předmět: |
Immunology
Osteoclasts chemical and pharmacologic phenomena Biology Hybrid Cells Mice Antigen Antibody Specificity Animals Humans Secretion Antigens Antibody-Producing Cells Molecular Biology Cells Cultured Lymphokines Lymphokine Molecular biology In vitro Immunization IL1A Immunoglobulin M Cell culture biology.protein Antibody Spleen Plasmacytoma |
| Zdroj: | Molecular immunology. 17(5) |
| ISSN: | 0161-5890 |
| Popis: | A system is described for production of hybridomas which secrete antibodies against the lymphokine osteoclast activating factor (OAF). The system consists of immunization of mouse spleen cells in vitro with purified or semipurified human OAF, followed by hybridization of the immunized cells with a mouse plasmacytoma cell line. The system for in vitro immunization includes conditioned medium from mouse thymus cells, which was shown to be a substantial aid to production of antigen-specific hybridomas, although the specific factors responsible for this enhancement were not determined. Using the system described, we were able to produce hybridomas with the desired antibody specificity using nanogram quantities of OAF as antigen. This system should allow greater flexibility and speed for the hybridoma technique, especially when applied to antigens which are scarce or impure, such as lymphokines. |
| Databáze: | OpenAIRE |
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