The Chemokine, Eotaxin, Activates Guinea-Pig Eosinophils in Vitro and Causes Their Accumulation into the Lung in Vivo
| Autor: | A.G. Rossi, Timothy J. Williams, David A. Griffiths-Johnson, Peter Collins, Peter J. Jose |
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| Rok vydání: | 1993 |
| Předmět: |
Eotaxin
Chemokine Neutrophils medicine.medical_treatment Guinea Pigs Biophysics Endogeny Biochemistry immune system diseases In vivo medicine Animals Humans Chemokine CCL5 Lung Molecular Biology Chromatography High Pressure Liquid Cell Aggregation Lymphokines Sequence Homology Amino Acid medicine.diagnostic_test biology Chemistry Cell Biology Allergens respiratory system Eosinophil Chromatography Ion Exchange In vitro respiratory tract diseases Eosinophils Bronchoalveolar lavage medicine.anatomical_structure Cytokine Immunology biology.protein Calcium Bronchoalveolar Lavage Fluid |
| Zdroj: | Biochemical and Biophysical Research Communications. 197:1167-1172 |
| ISSN: | 0006-291X |
| Popis: | Eotaxin is a novel C-C chemokine purified from the bronchoalveolar lavage (BAL) fluid of actively sensitised guinea-pigs after aerosol allergen challenge. In this study we show that eotaxin induced a dose-dependent increase in both intracellular-free calcium concentration ([Ca2+]i) and aggregation of guinea-pig eosinophils in vitro. Intradermally injected eotaxin induced the accumulation of [111In]eosinophils in naive guinea-pig skin in vivo, without oedema-inducing activity; the latter emerging in separate BAL fluid HPLC fractions. Aerosol exposure of naive guinea-pigs to eotaxin in vivo caused a selective increase in eosinophils in BAL fluid. Thus, eotaxin activates guinea-pig eosinophils in vitro and causes a selective eosinophil accumulation in the lung in vivo. Eotaxin and related molecules are potentially important endogenous signalling substances in allergic reactions in vivo. |
| Databáze: | OpenAIRE |
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