Maleylated human serum albumin inhibits HIV-1 infection in vitro
Autor: | Toshio Sone, Kohsuke Kino, Masao Takami, Tsunoo Hajime, Kenji Mizumoto |
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Rok vydání: | 1992 |
Předmět: |
CD4-Positive T-Lymphocytes
Clone (cell biology) Serum albumin Serum Albumin Human Biology Carrageenan Cell Line Cell Fusion Polysaccharides medicine Humans Binding site Receptor Molecular Biology Serum Albumin Syncytium Acquired Immunodeficiency Syndrome Binding Sites Binding protein Dextran Sulfate Maleates RNA-Directed DNA Polymerase Human serum albumin In vitro HIV Reverse Transcriptase Biochemistry biology.protein HIV-1 Molecular Medicine medicine.drug |
Zdroj: | Biochimica et biophysica acta. 1180(2) |
ISSN: | 0006-3002 |
Popis: | Maleylated-human serum albumin (Mal-HSA) inhibited human immunodeficiency virus type-1 (HIV-1) infection of MT-4 cells in vitro. It was also found to inhibit the fusion between uninfected CD4+ cells (Molt-4 clone 8 cells) and HIV-1 infected cells (Molt-4/HIV-1) to form syncytia. To investigate the mechanism of the inhibition, a study was designed to determine whether Mal-HSA could bind to CD4+ cells. Mal-HSA could bind to both MT-4 cells and Molt-4 clone 8 cells with high affinity, Kd = 2.0 nM and Kd = 5.8 nM, respectively. However, Mal-HSA could neither inhibit anti CD4 antibody Leu 3a binding to Molt-4 clone 8 cells nor modulate the expression of CD4 molecules on the surface of the cells. Mal-HSA binding to Molt-4 clone 8 cells was completely inhibited by sulfated polysaccharides bearing anti-HIV activity, such as dextran sulfate, fucoidan and carrageenan. Other HIV-1 susceptible human T-cell lines, such as Molt-4, CEM-5, H-9 and HuT-78 cells, also have Mal-HSA binding sites showing a high affinity, Kd = 0.9 +/- 0.4 nM. Mal-HSA binding proteins of Molt-4 clone 8 cells were identified by ligand blotting as 155 and 220 kDa proteins. Unlike dextran sulfate, Mal-HSA could not inhibit reverse transcriptase activity of HIV-1. These results indicate that Mal-HSA inhibits HIV-1 infection and syncytia formation, and suggest that 155 and/or 220 kDa proteins of target cells are involved in HIV-1 adsorption and/or the membrane fusion between HIV-1 and target cells. |
Databáze: | OpenAIRE |
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