Lung-resident eosinophils represent a distinct regulatory eosinophil subset
| Autor: | Thibaut Janss, Marc Radermecker, Florence Schleich, Xue Xiao, Thomas Marichal, Philipp Starkl, Monique Henket, Dimitri Pirottin, Claire Mesnil, Kris Thielemans, Laurent Gillet, Maria G. Belvisi, Fabrice Bureau, Renaud Louis, Marc Thiry, Mark A. Birrell, Stéfanie Raulier, Eve Ramery, Christophe Desmet, Geneviève Paulissen |
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| Přispěvatelé: | Physiology, Basic (bio-) Medical Sciences, Laboratory of Molecullar and Cellular Therapy, Immunomodulation in Chronic Inflammatory Diseases |
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Allergy Pathology medicine.medical_specialty Immunology Cell Inflammation Biology 03 medical and health sciences 0302 clinical medicine Immune system Antigen medicine Lung 11 Medical And Health Sciences General Medicine Eosinophil respiratory system medicine.disease respiratory tract diseases 030104 developmental biology medicine.anatomical_structure medicine.symptom Homeostasis 030215 immunology |
| Popis: | Increases in eosinophil numbers are associated with infection and allergic diseases, including asthma, but there is also evidence that eosinophils contribute to homeostatic immune processes. In mice, the normal lung contains resident eosinophils (rEos), but their function has not been characterized. Here, we have reported that steady-state pulmonary rEos are IL-5-independent parenchymal Siglec-FintCD62L+CD101lo cells with a ring-shaped nucleus. During house dust mite-induced airway allergy, rEos features remained unchanged, and rEos were accompanied by recruited inflammatory eosinophils (iEos), which were defined as IL-5-dependent peribronchial Siglec-FhiCD62L-CD101hi cells with a segmented nucleus. Gene expression analyses revealed a more regulatory profile for rEos than for iEos, and correspondingly, mice lacking lung rEos showed an increase in Th2 cell responses to inhaled allergens. Such elevation of Th2 responses was linked to the ability of rEos, but not iEos, to inhibit the maturation, and therefore the pro-Th2 function, of allergen-loaded DCs. Finally, we determined that the parenchymal rEos found in nonasthmatic human lungs (Siglec-8+CD62L+IL-3Rlo cells) were phenotypically distinct from the iEos isolated from the sputa of eosinophilic asthmatic patients (Siglec-8+CD62LloIL-3Rhi cells), suggesting that our findings in mice are relevant to humans. In conclusion, our data define lung rEos as a distinct eosinophil subset with key homeostatic functions. |
| Databáze: | OpenAIRE |
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