Dried blood spots perform well to identify patients with active HCV infection in Vietnam
Autor: | Thai Minh Nguyen, Binh T. Nguyen, Yoann Madec, Fabien Taieb, Trang Thi Thu Nguyen, Tram Thi Phuong Pham, Tuan Anh Nguyen, Hong Thi Bich Mai, Tram Tran, Mohand Ait-Ahmed, Huong Thi Thu Phan, Nhan Thi Do, Hien Ba Pham |
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Přispěvatelé: | National Institute of Hygiene and Epidemiology [Hanoi, Vietnam] (NIHE), Réseau International des Instituts Pasteur (RIIP), Nam Tu Liem District Medical Center [Hanoi], Dong Da General Hospital [Hanoi], Ministry of Health [Hanoi], Centre de Recherche Translationnelle - Center for Translational Science (CRT), Institut Pasteur [Paris], Epidémiologie des Maladies Emergentes - Emerging Diseases Epidemiology, Pasteur-Cnam Risques infectieux et émergents (PACRI), Institut Pasteur [Paris]-Conservatoire National des Arts et Métiers [CNAM] (CNAM)-Institut Pasteur [Paris]-Conservatoire National des Arts et Métiers [CNAM] (CNAM), We would like to thank Abbott Molecular Inc. for providing Abbott RealTime HCV assay reagents, consumables and funding for the study., Institut Pasteur [Paris] (IP), Institut Pasteur [Paris] (IP)-Conservatoire National des Arts et Métiers [CNAM] (CNAM), HESAM Université - Communauté d'universités et d'établissements Hautes écoles Sorbonne Arts et métiers université (HESAM)-HESAM Université - Communauté d'universités et d'établissements Hautes écoles Sorbonne Arts et métiers université (HESAM)-Institut Pasteur [Paris] (IP)-Conservatoire National des Arts et Métiers [CNAM] (CNAM), HESAM Université - Communauté d'universités et d'établissements Hautes écoles Sorbonne Arts et métiers université (HESAM)-HESAM Université - Communauté d'universités et d'établissements Hautes écoles Sorbonne Arts et métiers université (HESAM) |
Rok vydání: | 2020 |
Předmět: |
hepatitis C virus
medicine.medical_specialty Hepatitis C virus specificity Hepacivirus medicine.disease_cause Antiviral Agents Sensitivity and Specificity Gastroenterology Specimen Handling Central laboratory Limited access 03 medical and health sciences 0302 clinical medicine Virology Internal medicine Need treatment Humans Medicine 030212 general & internal medicine Dried blood Hepatology business.industry virus diseases Venous blood sensitivity Hepatitis C digestive system diseases Confidence interval viral load 3. Good health Infectious Diseases Vietnam RNA Viral [SDV.SPEE]Life Sciences [q-bio]/Santé publique et épidémiologie 030211 gastroenterology & hepatology Dried Blood Spot Testing business Viral load |
Zdroj: | Journal of Viral Hepatitis Journal of Viral Hepatitis, Wiley-Blackwell, 2020, 27 (5), pp.514-519. ⟨10.1111/jvh.13263⟩ Journal of Viral Hepatitis, 2020, 27 (5), pp.514-519. ⟨10.1111/jvh.13263⟩ |
ISSN: | 1365-2893 1352-0504 |
Popis: | International audience; Recently, treatment advances in direct-acting antivirals have radically changed the management of HCV patients. However, in resource-limited countries, identification of patients with active HCV infection is still challenging in remote settings due to the limited access to laboratories able to measure HCV viral load. This study evaluated whether dried blood spots (DBS) transferred to a central laboratory could overcome this challenge. A total of 315 HCV-infected patients, naïve to anti-HCV treatment, provided each three type of samples: plasma, DBS with calibrated quantities of venous blood and DBS with uncalibrated quantities of capillary blood. Qualitative comparison was conducted in terms of detection of HCV viral load on DBS as opposed to plasma to estimate sensitivity and specificity. Quantitative comparisons were conducted by means of correlation estimation. Of the 250 patients with detected plasma HCV viral load, 245 also had detectable DBS HCV viral load (capillary or venous) leading to a sensitivity of 98.0% (95% confidence interval (CI): 95.4%-99.3%); importantly, all measurements with a plasma HCV viral load >118 IU/mL were also detected in DBS. When HCV was not detected in plasma, it was also not detected in DBS resulting in 100% specificity (95% CI: 94.5%-100%). Quantitative HCV viral load results were very similar when utilizing plasma or DBS sample types as illustrated by correlations >0.99. In conclusion, DBS sample types, with either uncalibrated capillary blood or calibrated venous blood, performed well to distinguish patients with active HCV infection, and who therefore need treatment, from other patients. |
Databáze: | OpenAIRE |
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