Characterization ofN-acyl homoserine lactone overproducing mutants ofBurkholderia multivoransATCC 17616
| Autor: | Thomas G. Lessie, Hongwei Zhou, Fude Yao |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
Burkholderia
Molecular Sequence Data Mutant Homoserine Biology Microbiology Ligases chemistry.chemical_compound 4-Butyrolactone Bacterial Proteins Genetics Amino Acid Sequence Selection Genetic Overproduction Molecular Biology Gene Burkholderia multivorans food and beverages biochemical phenomena metabolism and nutrition biology.organism_classification N-Acyl homoserine lactone Biochemistry chemistry Genes Bacterial Mutation Trans-Activators Biological Assay Chromobacterium violaceum |
| Zdroj: | FEMS Microbiology Letters. 206:201-207 |
| ISSN: | 1574-6968 0378-1097 |
| DOI: | 10.1111/j.1574-6968.2002.tb11010.x |
| Popis: | Burkholderia multivorans ATCC 17616 ordinarily produces insufficient amounts of N-acyl homoserine lactones (AHLs) to promote AHL-dependent formation of the pigment violacein by the reporter strain Chromobacterium violaceum CV026. We have isolated AHL-overproducing mutants of strain 17616 by screening for variants which do cross-feed AHLs to strain CV026. Nucleotide-sequence analysis of the bmuIR locus which specifies AHL synthase (BmuI) and AHL-binding transcriptional activator protein (BmuR) indicated that the increased capacity to produce AHLs was not a consequence of changes upstream or internal to the bmuI or bmuR genes. We conclude that the mutations leading to AHL overproduction lie outside the bmuI/bmuR locus. |
| Databáze: | OpenAIRE |
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