| Autor: |
Rahul Krishnan, Rahul Rajendran, Yo-Seb Jang, Jong-Oh Kim, Su-Young Yoon, Myung-Joo Oh |
| Rok vydání: |
2022 |
| Předmět: |
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| Zdroj: |
Fish & Shellfish Immunology. 127:219-227 |
| ISSN: |
1050-4648 |
| DOI: |
10.1016/j.fsi.2022.06.026 |
| Popis: |
NLRC3 is identified as a unique regulatory NLR involved in the modulation of cellular processes and inflammatory responses. In this study, a novel Nod like receptor C3 (NLRC3) was functionally characterized from seven band grouper in the context of nervous necrosis virus infection. The grouper NLRC3 is highly conserved and homologous with other vertebrate proteins with a NACHT domain and a C-terminal leucine-rich repeat (LRR) domain and an N-terminal CARD domain. Quantitative gene expression analysis revealed the highest mRNA levels of NLRC3 were in the brain and gill followed by the spleen and kidney following NNV infection. Overexpression of NLRC3 augmented the NNV replication kinetics in primary grouper brain cells. NLRC3 attenuated the interferon responses in the cells following NNV infection by impacting the TRAF6/NF-κB activity and exhibited reduced IFN sensitivity, ISRE promoter activity, and IFN pathway gene expression. In contrast, NLRC3 expression positively regulated the inflammasome response and pro-inflammatory gene expression during NNV infection. NLRC3 negatively regulates the PI3K-mTOR axis and activated the cellular autophagic response. Delineating the complexity of NLRC3 regulation of immune response in the primary grouper brain cells following NNV infection suggests that the protein acts as a virally manipulated host factor that negatively regulated the antiviral immune response to augment the NNV replication. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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Full Text from ScienceDirect