A light-initiated, dark oxidation of bacteriochlorophyll from reaction center particles
| Autor: | Daniel C. Brune, Roderick K. Clayton |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
Photosynthetic reaction centre
Strain (chemistry) Light Extraction (chemistry) Detergents Photosynthetic Reaction Center Complex Proteins Biophysics Cell Biology Darkness Hydrogen-Ion Concentration Photochemistry Rhodopseudomonas spheroides Biochemistry Oxygen chemistry.chemical_compound Dark interval Rhodopseudomonas chemistry Lauryldimethylamine oxide Methanol Bacteriochlorophyll Bacteriochlorophylls Oxidation-Reduction Dimethylamines |
| Zdroj: | Biochimica et biophysica acta. 333(1) |
| ISSN: | 0006-3002 |
| Popis: | When reaction center particles from Rhodopseudomonas spheroides strain R26 are illuminated and then extracted with methanol, about one-third of the extracted bacteriochlorophyll slowly becomes oxidized, The oxidation does not occur under anaerobic conditions or in the absence of the detergent lauryldimethylamine oxide. Alkaline conditions also prevent the reaction. A dark interval between illumination and extraction delays the onset of bacteriochlorophyll oxidation in a predictable way. These results are consistent with the hypothesis that illumination generates a reaction initiator which is fairly stable in methanol but decays with a half-life of about 4.5 min in reaction center particles after illumination ceases. |
| Databáze: | OpenAIRE |
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