Monitoring genomic alterations with a panel of oligonucleotide probes specific for various simple repeat motifs
| Autor: | Eike Fuhrmann, H. Pöche, Gundula Thiel, Tanka Losanova, Peter Nürnberg, Ingrid Barth, Christian Peters, Claudia Lenzner |
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| Rok vydání: | 1991 |
| Předmět: |
Male
Skull Neoplasms Clinical Biochemistry Biology medicine.disease_cause Biochemistry Genome Germline Analytical Chemistry medicine Humans Lymphocytes Repeated sequence Repetitive Sequences Nucleic Acid Genetics Mutation Base Sequence Oligonucleotide Glioma DNA Fingerprinting Pedigree Genetic marker Female Human genome Oligonucleotide Probes Molecular probe |
| Zdroj: | Electrophoresis. 12:186-192 |
| ISSN: | 1522-2683 0173-0835 |
| DOI: | 10.1002/elps.1150120215 |
| Popis: | Germline and somatic instability of the human genome was studied, using synthetic oligonucleotides specific for simple repeat motifs. The following probes were used: (GTG)5, (GACA)4, (GATA)4, (CT)8, (TTAGGG)3, (GT)8, (GAA)6 and (GGAT)4. Each of them is unique with respect to the target regions recognized in the genome. Thus compilation of the various fingerprint data provides a complex map of the genome (and its deviations). While the fingerprints of differentiated somatic tissues never showed any alterations, in tumor tissues (namely gliomas) many changes could be detected. Most of the latter reflect secondary karyological aberrations. In nearly one third of the gliomas, drastically amplified and apparently monomorphic DNA fragments were identified. This marker should make it possible to deal with causal pathogenetic mechanisms as well as novel diagnostic strategies. |
| Databáze: | OpenAIRE |
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