Loss of mitochondrial membrane potential is not essential to hepatocyte killing by allyl alcohol
| Autor: | Cai Dy, Hornbook Kr, Lora E. Rikans |
|---|---|
| Rok vydání: | 1995 |
| Předmět: |
Male
Propanols Mitochondria Liver 1-Propanol Mitochondrion Toxicology Rhodamine 123 Dithiothreitol Membrane Potentials chemistry.chemical_compound Cyclosporin a medicine Animals Allyl alcohol Membrane potential Cell Death L-Lactate Dehydrogenase Chemistry organic chemicals food and beverages General Medicine Rats Inbred F344 Trifluoperazine Rats medicine.anatomical_structure Cell killing Liver Biochemistry Hepatocyte Cyclosporine Biophysics |
| Zdroj: | Toxicology Letters. 81:159-165 |
| ISSN: | 0378-4274 |
| DOI: | 10.1016/0378-4274(95)03426-9 |
| Popis: | Allyl alcohol-induced LDH leakage from isolated rat hepatocytes was preceded by a decrease in rhodamine 123 retention, signifying a loss of mitochondrial membrane potential. Addition of dithiothreitol (DTT) prevented the drop in membrane potential and completely prevented cell killing by allyl alcohol. In contrast, cyclosporin A and trifluoperazine delayed the loss of membrane potential without affecting cytolethality. The results indicate that a drop in mitochondrial membrane potential is not essential for allyl alcohol lethality. The mitochondrial dysfunction produced by allyl alcohol appears to be the consequence of an earlier event in the toxicity that is reversible by DTT. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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