Cytochrome P450 isozymes involved in the metabolism of phenol, a benzene metabolite

Autor:	Mark W Powley, Gary P. Carlson
Rok vydání:	2001
Předmět:	Male Unspecific monooxygenase Phenol Hydroquinone Metabolite Cytochrome P450 Benzene Cytochrome P-450 CYP2E1 General Medicine Biology CYP2E1 Toxicology Isozyme Mice chemistry.chemical_compound Cytochrome P-450 Enzyme System chemistry Biochemistry Microsomes Microsome biology.protein Animals
Zdroj:	Toxicology Letters. 125:117-123
ISSN:	0378-4274
DOI:	10.1016/s0378-4274(01)00441-6
Popis:	Benzene is an occupational and environmental toxicant. The major health concern for humans is acute myelogenous leukemia. To exert its toxic effects, benzene must be metabolized by cytochrome P450 to phenol and subsequently to catechol and hydroquinone. Previous research has implicated CYP2E1 in the metabolism of phenol. In this study the cytochrome P450 isozymes involved in the metabolism of phenol were examined in hepatic and pulmonary microsomes utilizing chemical inhibitors of CYP2E1, CYP2B, and CYP2F2 and using CYP2E1 knockout mice. CYP2E1 was found to be responsible for only approximately 50% of 20 microM phenol metabolism in the liver. This suggests another isozyme(s) is involved in hepatic phenol metabolism. In pulmonary microsomes both CYP2E1 and CYP2F2 were significantly involved.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f0046a2807ee09a6907300d3cb634bf6 https://doi.org/10.1016/s0378-4274(01)00441-6 Zobrazit plný text záznamu Full Text from ScienceDirect